Digital holographic microscopy: A noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy

Pierre Marquet*, Benjamin Rappaz, Pierre J. Magistretti, Etienne Cuche, Yves Emery, Tristan Colomb, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1119 Scopus citations


We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.

Original languageEnglish (US)
Pages (from-to)468-470
Number of pages3
JournalOptics Letters
Issue number5
StatePublished - Mar 1 2005
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics

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