TY - JOUR
T1 - Digital holographic microscopy
T2 - A noninvasive contrast imaging technique allowing quantitative visualization of living cells with subwavelength axial accuracy
AU - Marquet, Pierre
AU - Rappaz, Benjamin
AU - Magistretti, Pierre J.
AU - Cuche, Etienne
AU - Emery, Yves
AU - Colomb, Tristan
AU - Depeursinge, Christian
PY - 2005/3/1
Y1 - 2005/3/1
N2 - We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.
AB - We have developed a digital holographic microscope (DHM), in a transmission mode, especially dedicated to the quantitative visualization of phase objects such as living cells. The method is based on an original numerical algorithm presented in detail elsewhere [Cuche et al., Appl. Opt. 38, 6994 (1999)]. DHM images of living cells in culture are shown for what is to our knowledge the first time. They represent the distribution of the optical path length over the cell, which has been measured with subwavelength accuracy. These DHM images are compared with those obtained by use of the widely used phase contrast and Nomarski differential interference contrast techniques.
UR - http://www.scopus.com/inward/record.url?scp=14844322300&partnerID=8YFLogxK
U2 - 10.1364/OL.30.000468
DO - 10.1364/OL.30.000468
M3 - Article
C2 - 15789705
AN - SCOPUS:14844322300
SN - 0146-9592
VL - 30
SP - 468
EP - 470
JO - OPTICS LETTERS
JF - OPTICS LETTERS
IS - 5
ER -