Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics

Pierre Marquet; Benjamin Rappaz; Tristan Colomb; Florian Charrière; Jonas Kühn; Yves Emery; Etienne Cuche; Christian Depeursinge; Pierre Magistretti

doi:10.1117/12.662988

Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics

Pierre Marquet^*, Benjamin Rappaz, Tristan Colomb, Florian Charrière, Jonas Kühn, Yves Emery, Etienne Cuche, Christian Depeursinge, Pierre Magistretti

^*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

5 Scopus citations

Abstract

We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

Original language	English (US)
Title of host publication	Biophotonics and New Therapy Frontiers
DOIs	https://doi.org/10.1117/12.662988
State	Published - 2006
Externally published	Yes
Event	Biophotonics and New Therapy Frontiers - Strasbourg, France Duration: Apr 3 2006 → Apr 5 2006

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	6191
ISSN (Print)	1605-7422

Other

Other	Biophotonics and New Therapy Frontiers
Country/Territory	France
City	Strasbourg
Period	04/3/06 → 04/5/06

Keywords

3D cellular dynamics
Digital Holography Microscopy
Intracellular refractive index

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Radiology Nuclear Medicine and imaging
Biomaterials

Access to Document

10.1117/12.662988

Cite this

Marquet, P., Rappaz, B., Colomb, T., Charrière, F., Kühn, J., Emery, Y., Cuche, E., Depeursinge, C., & Magistretti, P. (2006). Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics. In Biophotonics and New Therapy Frontiers Article 61910U (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6191). https://doi.org/10.1117/12.662988

@inproceedings{474d4928b45341789187dd428cfac190,

title = "Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics",

abstract = "We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.",

keywords = "3D cellular dynamics, Digital Holography Microscopy, Intracellular refractive index",

author = "Pierre Marquet and Benjamin Rappaz and Tristan Colomb and Florian Charri{\`e}re and Jonas K{\"u}hn and Yves Emery and Etienne Cuche and Christian Depeursinge and Pierre Magistretti",

year = "2006",

doi = "10.1117/12.662988",

language = "English (US)",

isbn = "0819462470",

series = "Progress in Biomedical Optics and Imaging - Proceedings of SPIE",

booktitle = "Biophotonics and New Therapy Frontiers",

note = "Biophotonics and New Therapy Frontiers ; Conference date: 03-04-2006 Through 05-04-2006",

}

Marquet, P, Rappaz, B, Colomb, T, Charrière, F, Kühn, J, Emery, Y, Cuche, E, Depeursinge, C & Magistretti, P 2006, Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics. in Biophotonics and New Therapy Frontiers., 61910U, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 6191, Biophotonics and New Therapy Frontiers, Strasbourg, France, 04/3/06. https://doi.org/10.1117/12.662988

Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics. / Marquet, Pierre; Rappaz, Benjamin; Colomb, Tristan et al.
Biophotonics and New Therapy Frontiers. 2006. 61910U (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 6191).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics

AU - Marquet, Pierre

AU - Rappaz, Benjamin

AU - Colomb, Tristan

AU - Charrière, Florian

AU - Kühn, Jonas

AU - Emery, Yves

AU - Cuche, Etienne

AU - Depeursinge, Christian

AU - Magistretti, Pierre

PY - 2006

Y1 - 2006

N2 - We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

AB - We have developed a digital holographic microscope (DHM), in a transmission mode, adapted to the quantitative study of cellular dynamics. Living cells are optically probed by measuring the phase shift they produce on the transmitted wave front. The high temporal stability of the phase signal, equivalent to λ/1800, and the low acquisition time (down to 20 μs) enables to monitor cellular dynamics processes. An experimental procedure allowing to calculate both the intracellular refractive index and the cellular thickness (morphometry) from the measured phase shift is presented.

KW - 3D cellular dynamics

KW - Digital Holography Microscopy

KW - Intracellular refractive index

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U2 - 10.1117/12.662988

DO - 10.1117/12.662988

M3 - Conference contribution

AN - SCOPUS:33745132405

SN - 0819462470

SN - 9780819462473

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biophotonics and New Therapy Frontiers

T2 - Biophotonics and New Therapy Frontiers

Y2 - 3 April 2006 through 5 April 2006

ER -

Digital holographic microscopy, a new optical imaging technique to investigate cellular dynamics

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Keywords

ASJC Scopus subject areas

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