The molecular techniques of in situ transcription and antisense RNA amplification (IST/aRNA) have allowed for the monitoring of coordinate changes in the expression of multiple genes simultaneously. However, the analysis of their concurrent behavior during murine embryogenesis has been problematic. Studies involving the investigation of temporal and spatial gene expression during embryogenesis have focused solely on the analysis of isolated, single gene events. Such an approach has failed to provide an integrative picture of genetic control over the varied and complicated cellular processes governing embryogenesis. In order to interpret the enormous amount of gene expression data generated by these procedures, we have attempted to develop an analytical framework by employing the statistical concepts of principal components analysis (PCA). For the current study, we performed IST/aRNA on neural tubes dissected from the highly inbred LM/Bc murine strain collected during four gestational time periods. A subset of these genes, representing a partial signaling pathway in the developing neuroepithelium, was then subjected to PCA. Here, we report that PCA highlighted the transcriptional interplay among the genes p53, wee-1, Tgfβ- 2, and bcl-2 such that the combined reciprocal regulation of their gene products is suggestive of a predominant proliferative state for the developing neuroepithelium. The application of PCA to the gene expression data has elucidated previously unknown interrelationships among cell cycle genes, growth, and transcription factors on a transcriptional level during critical stages of neurulation. The information gleaned from this analysis, while not definitive, suggests distinct hypotheses to guide future research.
Bibliographical noteFunding Information:
This work was supported in part by Grants DE 11303 and ES 07165 from the National Institutes of Health. The authors express their appreciation to Ms. M. Wheeler and Ms. L. Cook for the well-being of the mice, Ms. M. Davda for her technical expertise, and Ms. A. Miller for her efficient editorial assistance. We thank the following individuals for providing cDNA clones used in these experiments: M. Pisano, G. Wilson, S. Korsmeyer, F. Ruddle, D. Epstein, R. Harland, T. Luikin, A. Brown, S. Miyatani, M. Wrzos, A. McMahon, and J. Eberwine.
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