Developmental competence of porcine genome-edited zygotes

Maria A. Gil; Cristina A. Martinez; Alicia Nohalez; Inmaculada Parrilla; Jordi Roca; Jun Wu; Pablo J. Ross; Cristina Cuello; Juan C. Izpisua; Emilio A. Martinez

doi:10.1002/mrd.22829

Developmental competence of porcine genome-edited zygotes

Maria A. Gil^*, Cristina A. Martinez, Alicia Nohalez, Inmaculada Parrilla, Jordi Roca, Jun Wu, Pablo J. Ross, Cristina Cuello, Juan C. Izpisua, Emilio A. Martinez

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Review article › peer-review

11 Scopus citations

Abstract

Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

Original language	English (US)
Pages (from-to)	814-821
Number of pages	8
Journal	Molecular Reproduction and Development
Volume	84
Issue number	9
DOIs	https://doi.org/10.1002/mrd.22829
State	Published - Sep 2017

Bibliographical note

Publisher Copyright:
© 2017 Wiley Periodicals, Inc.

Keywords

CRISPR/Cas9
TALEN
ZFN
cytoplasmic microinjection
in vitro-produced zygotes
in vivo-derived zygotes

ASJC Scopus subject areas

Genetics
Cell Biology
Developmental Biology

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10.1002/mrd.22829

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title = "Developmental competence of porcine genome-edited zygotes",

abstract = "Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.",

keywords = "CRISPR/Cas9, TALEN, ZFN, cytoplasmic microinjection, in vitro-produced zygotes, in vivo-derived zygotes",

author = "Gil, {Maria A.} and Martinez, {Cristina A.} and Alicia Nohalez and Inmaculada Parrilla and Jordi Roca and Jun Wu and Ross, {Pablo J.} and Cristina Cuello and Izpisua, {Juan C.} and Martinez, {Emilio A.}",

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doi = "10.1002/mrd.22829",

language = "English (US)",

volume = "84",

pages = "814--821",

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TY - JOUR

T1 - Developmental competence of porcine genome-edited zygotes

AU - Gil, Maria A.

AU - Martinez, Cristina A.

AU - Nohalez, Alicia

AU - Parrilla, Inmaculada

AU - Roca, Jordi

AU - Wu, Jun

AU - Ross, Pablo J.

AU - Cuello, Cristina

AU - Izpisua, Juan C.

AU - Martinez, Emilio A.

PY - 2017/9

Y1 - 2017/9

N2 - Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

AB - Genome editing in pigs has tremendous practical applications for biomedicine. The advent of genome editing technology, with its use of site-specific nucleases—including ZFNs, TALENs, and the CRISPR/Cas9 system—has popularized targeted zygote genome editing via one-step microinjection in several mammalian species. Here, we review methods to optimize the developmental competence of genome-edited porcine embryos and strategies to improve the zygote genome-editing efficiency in pigs.

KW - CRISPR/Cas9

KW - TALEN

KW - ZFN

KW - cytoplasmic microinjection

KW - in vitro-produced zygotes

KW - in vivo-derived zygotes

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U2 - 10.1002/mrd.22829

DO - 10.1002/mrd.22829

M3 - Review article

C2 - 28471514

AN - SCOPUS:85020307113

SN - 1040-452X

VL - 84

SP - 814

EP - 821

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

IS - 9

ER -

Developmental competence of porcine genome-edited zygotes

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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