Development, optimization and evaluation of long chain nanolipid carrier for hepatic delivery of silymarin through lymphatic transport pathway

Shilpa Chaudhary, Tarun Garg, R. S.R. Murthy, Goutam Rath, Amit K. Goyal

Research output: Contribution to journalArticlepeer-review

49 Scopus citations

Abstract

In the present study, nanostructured lipid carriers (NLCs) with three different lipid combinations (solid lipid:liquid lipid) were prepared through emulsification and ultrasonication using a Box-Behnken design. From the design, the best lipid combination was glyceryl monostearate and oleic acid, which gives particle of smaller size (223.73 ± 43.39 nm) with high drug entrapment efficiency (78.65 ± 2.2%). In vitro release studies show that 84.60 ± 5.66% of drug was released in 24 h. In vivo studies revealed that drug absorption occurs through lymphatic pathway as only 5.008 ± 0.011 μg/ml of peak plasma concentration was achieved in blood plasma in presence of chylomicron inhibitor. The peak plasma concentration (Cmax) for silymarin loaded NLC was found to be 25.565 ± 0.969 μg/ml as compared to silymarin suspension whose Cmax was found to be 14.050 ± 0.552 μg/ml, this confirms 2-fold increase in relative bioavailability. In vivo studies revealed that 19.268 ± 1.29 μg of drug reaches to liver in 2 h whereas negligible drug concentration reported in other organs. It was concluded that drug loaded NLCs was beneficial for targeting liver or other lymphatic disorders through lymphatic transport pathway. Finally, the main purpose of modifying lymphatic transport system was successfully achieved through NLCs.
Original languageEnglish (US)
Pages (from-to)108-121
Number of pages14
JournalInternational Journal of Pharmaceutics
Volume485
Issue number1-2
DOIs
StatePublished - May 15 2015
Externally publishedYes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-10-12

Fingerprint

Dive into the research topics of 'Development, optimization and evaluation of long chain nanolipid carrier for hepatic delivery of silymarin through lymphatic transport pathway'. Together they form a unique fingerprint.

Cite this