Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates

Shuho Nozue; Satoshi Habuchi

doi:10.1117/12.3023289

Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

We propose a new method to engineer point spread function (PSF) for 3D single-particle tracking using mica. The imaging system in this method uses a standard wide-field fluorescence microscope; the only difference from the ordinary is using a mica as a substrate to mount the sample instead of the coverslip. This approach would have advantages over easy-toimplement, wide axial range tracking capability, and multiplexity of tracking. We demonstrate 3D single-particle tracking in a homogeneous solution.

Original language	English (US)
Title of host publication	Three-Dimensional and Multidimensional Microscopy
Subtitle of host publication	Image Acquisition and Processing XXXI
Editors	Thomas G. Brown, Tony Wilson, Laura Waller
Publisher	SPIE
ISBN (Electronic)	9781510669550
DOIs	https://doi.org/10.1117/12.3023289
State	Published - 2024
Event	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI 2024 - San Francisco, United States Duration: Jan 31 2024 → Feb 1 2024

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12848
ISSN (Print)	1605-7422

Conference

Conference	Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI 2024
Country/Territory	United States
City	San Francisco
Period	01/31/24 → 02/1/24

Bibliographical note

Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

Keywords

Birefringence
Fluorescence imaging
PSF engineering
Single-particle tracking
Three-dimensional

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.3023289

Cite this

Nozue, S., & Habuchi, S. (2024). Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates. In T. G. Brown, T. Wilson, & L. Waller (Eds.), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI Article 128480F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12848). SPIE. https://doi.org/10.1117/12.3023289

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Nozue, S & Habuchi, S 2024, Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates. in TG Brown, T Wilson & L Waller (eds), Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI., 128480F, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12848, SPIE, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI 2024, San Francisco, United States, 01/31/24. https://doi.org/10.1117/12.3023289

Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates. / Nozue, Shuho ; Habuchi, Satoshi.
Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI. ed. / Thomas G. Brown; Tony Wilson; Laura Waller. SPIE, 2024. 128480F (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12848).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

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Nozue S , Habuchi S. Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates. In Brown TG, Wilson T, Waller L, editors, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXXI. SPIE. 2024. 128480F. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.3023289

Development of long axial range 3D single-particle tracking by PSF engineering using birefringent substrates

Abstract

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Bibliographical note

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ASJC Scopus subject areas

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