TY - GEN
T1 - Development of a sensing system to detect C-telopeptide of type-I collagen
AU - Afsarimanesh, Nasrin
AU - Zia, Asif I.
AU - Mukhopadhyay, S. C.
AU - Kruger, Marlena
AU - Yu, P. L.
AU - Kosel, Jürgen
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2016/3/30
Y1 - 2016/3/30
N2 - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.
AB - This research work describes a non-invasive and label-free immunosensing technique to detect the C-telopeptide of type-I collagen (CTX-1) by Electrochemical Impedance Spectroscopy (EIS). A planar interdigital capacitive sensor is used to evaluate the properties of the material under test. This sensor was fabricated on the basis of thin film micro-electromechanical system (MEMS) semiconductor device fabrication technology. EIS was used in conjunction with the sensor to detect collagen type-I in blood plasma. At the first stage, the Serum CrossLaps® ELISA was used to measure some known samples in order to obtain a standard curve. Streptavidin agarose was successfully immobilized on the sensing area of the sensor. After that the experiments were done with antibody solution and three known samples of CTX-1, zero concentration which was considered as control, 2.669 ng/ml and 0.798 ng/ml concentration. The results are encouraging for further investigation.
UR - http://hdl.handle.net/10754/622533
UR - http://ieeexplore.ieee.org/document/7438466/
UR - http://www.scopus.com/inward/record.url?scp=84964814961&partnerID=8YFLogxK
U2 - 10.1109/ICSensT.2015.7438466
DO - 10.1109/ICSensT.2015.7438466
M3 - Conference contribution
SN - 9781479963140
SP - 586
EP - 590
BT - 2015 9th International Conference on Sensing Technology (ICST)
PB - Institute of Electrical and Electronics Engineers (IEEE)
ER -