Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.

Xiaomei Tang; Shulin Chen; Huiwen Yu; Xiongjie Zheng; Fei Zhang; Xiuxin Deng; Qiang Xu

doi:10.1007/s00299-021-02781-7

Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.

Xiaomei Tang, Shulin Chen, Huiwen Yu, Xiongjie Zheng, Fei Zhang, Xiuxin Deng, Qiang Xu

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Key messageHere, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.

Original language	English (US)
Journal	Plant cell reports
DOIs	https://doi.org/10.1007/s00299-021-02781-7
State	Published - Sep 23 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-09-27
Acknowledgements: We are grateful to Dr. Qi Xie from Institute of Genetics and Development, Chinese Academy of Sciences for providing the YAO promoter-driven CRISPR/Cas9 vector and Kabin Xie from College of Plant Sciences and Technology, Huazhong Agricultural University for the guidance of the vectors construction. This work was supported by the National Key Research and Development Program of China (NO. 2018YFD1000101), National Natural Science Foundation of China (NOs. 31925034 and 31872052), and the Fundamental Research Funds for the Central Universities.

ASJC Scopus subject areas

Plant Science
Agronomy and Crop Science

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10.1007/s00299-021-02781-7

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title = "Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.",

abstract = "Key messageHere, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.",

author = "Xiaomei Tang and Shulin Chen and Huiwen Yu and Xiongjie Zheng and Fei Zhang and Xiuxin Deng and Qiang Xu",

note = "KAUST Repository Item: Exported on 2021-09-27 Acknowledgements: We are grateful to Dr. Qi Xie from Institute of Genetics and Development, Chinese Academy of Sciences for providing the YAO promoter-driven CRISPR/Cas9 vector and Kabin Xie from College of Plant Sciences and Technology, Huazhong Agricultural University for the guidance of the vectors construction. This work was supported by the National Key Research and Development Program of China (NO. 2018YFD1000101), National Natural Science Foundation of China (NOs. 31925034 and 31872052), and the Fundamental Research Funds for the Central Universities.",

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month = sep,

day = "23",

doi = "10.1007/s00299-021-02781-7",

language = "English (US)",

journal = "Plant cell reports",

issn = "0721-7714",

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TY - JOUR

T1 - Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.

AU - Tang, Xiaomei

AU - Chen, Shulin

AU - Yu, Huiwen

AU - Zheng, Xiongjie

AU - Zhang, Fei

AU - Deng, Xiuxin

AU - Xu, Qiang

N1 - KAUST Repository Item: Exported on 2021-09-27 Acknowledgements: We are grateful to Dr. Qi Xie from Institute of Genetics and Development, Chinese Academy of Sciences for providing the YAO promoter-driven CRISPR/Cas9 vector and Kabin Xie from College of Plant Sciences and Technology, Huazhong Agricultural University for the guidance of the vectors construction. This work was supported by the National Key Research and Development Program of China (NO. 2018YFD1000101), National Natural Science Foundation of China (NOs. 31925034 and 31872052), and the Fundamental Research Funds for the Central Universities.

PY - 2021/9/23

Y1 - 2021/9/23

N2 - Key messageHere, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.

AB - Key messageHere, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.

UR - http://hdl.handle.net/10754/671938

UR - https://link.springer.com/10.1007/s00299-021-02781-7

U2 - 10.1007/s00299-021-02781-7

DO - 10.1007/s00299-021-02781-7

M3 - Article

C2 - 34554293

SN - 0721-7714

JO - Plant cell reports

JF - Plant cell reports

ER -

Development of a gRNA-tRNA array of CRISPR/Cas9 in combination with grafting technique to improve gene-editing efficiency of sweet orange.

Abstract

Bibliographical note

ASJC Scopus subject areas

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