Key messageHere, we developed a reliable protocol for the fast and efficient gene-edited Anliu sweet orange plants production. The application of in vitro shoot grafting technology significantly reduced the growth cycle of transgenic seedlings, and the survival rate of cleft grafting was more than 90%. In addition, the mutation efficiency of the grafted geneedited sweet orange was significantly improved by short-term heat stress treatments. Thus, the combination strategy of grafting and heat stress treatments provided a reference for the fast and efficient multiplex gene editing of sweet orange.
Bibliographical noteKAUST Repository Item: Exported on 2021-09-27
Acknowledgements: We are grateful to Dr. Qi Xie from Institute of Genetics and Development, Chinese Academy of Sciences for providing the YAO promoter-driven CRISPR/Cas9 vector and Kabin Xie from College of Plant Sciences and Technology, Huazhong Agricultural University for the guidance of the vectors construction. This work was supported by the National Key Research and Development Program of China (NO. 2018YFD1000101), National Natural Science Foundation of China (NOs. 31925034 and 31872052), and the Fundamental Research Funds for the Central Universities.
ASJC Scopus subject areas
- Plant Science
- Agronomy and Crop Science