Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications

Elisabetta Dattola; Elvira Immacolata Parrotta; Stefania Scalise; Gerardo Perozziello; Tania Limongi; Patrizio Candeloro; Maria Laura Coluccio; Carmine Maletta; Luigi Bruno; Maria Teresa De Angelis; Gianluca Santamaria; Vincenzo Mollace; Ernesto Lamanna; Enzo M. Di Fabrizio; Giovanni Cuda

doi:10.1039/c8ra08187e

Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications

Elisabetta Dattola, Elvira Immacolata Parrotta, Stefania Scalise, Gerardo Perozziello, Tania Limongi, Patrizio Candeloro, Maria Laura Coluccio, Carmine Maletta, Luigi Bruno, Maria Teresa De Angelis, Gianluca Santamaria, Vincenzo Mollace, Ernesto Lamanna, Enzo M. Di Fabrizio, Giovanni Cuda

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87 Scopus citations

Abstract

The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.

Original language	English (US)
Pages (from-to)	4246-4257
Number of pages	12
Journal	RSC Advances
Volume	9
Issue number	8
DOIs	https://doi.org/10.1039/c8ra08187e
State	Published - 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the project for Young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.

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Dattola, E., Parrotta, E. I., Scalise, S., Perozziello, G., Limongi, T., Candeloro, P., Coluccio, M. L., Maletta, C., Bruno, L., De Angelis, M. T., Santamaria, G., Mollace, V., Lamanna, E., Di Fabrizio, E. M., & Cuda, G. (2019). Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications. RSC Advances, 9(8), 4246-4257. https://doi.org/10.1039/c8ra08187e

@article{079ba426f9b94fb09a76d384b9d529aa,

title = "Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications",

abstract = "The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.",

author = "Elisabetta Dattola and Parrotta, {Elvira Immacolata} and Stefania Scalise and Gerardo Perozziello and Tania Limongi and Patrizio Candeloro and Coluccio, {Maria Laura} and Carmine Maletta and Luigi Bruno and {De Angelis}, {Maria Teresa} and Gianluca Santamaria and Vincenzo Mollace and Ernesto Lamanna and {Di Fabrizio}, {Enzo M.} and Giovanni Cuda",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the project for Young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.",

year = "2019",

doi = "10.1039/c8ra08187e",

language = "English (US)",

volume = "9",

pages = "4246--4257",

journal = "RSC Advances",

issn = "2046-2069",

publisher = "ROYAL SOC CHEMISTRY",

number = "8",

}

Dattola, E, Parrotta, EI, Scalise, S, Perozziello, G, Limongi, T, Candeloro, P, Coluccio, ML, Maletta, C, Bruno, L, De Angelis, MT, Santamaria, G, Mollace, V, Lamanna, E, Di Fabrizio, EM & Cuda, G 2019, 'Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications', RSC Advances, vol. 9, no. 8, pp. 4246-4257. https://doi.org/10.1039/c8ra08187e

TY - JOUR

T1 - Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications

AU - Dattola, Elisabetta

AU - Parrotta, Elvira Immacolata

AU - Scalise, Stefania

AU - Perozziello, Gerardo

AU - Limongi, Tania

AU - Candeloro, Patrizio

AU - Coluccio, Maria Laura

AU - Maletta, Carmine

AU - Bruno, Luigi

AU - De Angelis, Maria Teresa

AU - Santamaria, Gianluca

AU - Mollace, Vincenzo

AU - Lamanna, Ernesto

AU - Di Fabrizio, Enzo M.

AU - Cuda, Giovanni

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by the project for Young researchers financed from the Italian Ministry of Health “High throughput analysis of cancer cells for therapy evaluation by microfluidic platforms integrating plasmonic nanodevices” (CUP J65C13001350001, project No. GR-2010-2311677) granted to the nanotechnology laboratory of the Department of Experimental and Clinical Medicine of the University “Magna Graecia” of Catanzaro.

PY - 2019

Y1 - 2019

N2 - The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.

AB - The aim of this study was the design of a 3D scaffold composed of poly(vinyl) alcohol (PVA) for cardiac tissue engineering (CTE) applications. The PVA scaffold was fabricated using a combination of gas foaming and freeze-drying processes that did not need any cross-linking agents. We obtained a biocompatible porous matrix with excellent mechanical properties. We measured the stress–strain curves of the PVA scaffolds and we showed that the elastic behavior is similar to that of the extracellular matrix of muscles. The SEM observations revealed that the scaffolds possess micro pores having diameters ranging from 10 μm to 370 μm that fit to the dimensions of the cells. A further purpose of this study was to test scaffolds ability to support human induced pluripotent stem cells growth and differentiation into cardiomyocytes. As the proliferation tests show, the number of live stem cells on the scaffold after 12 days was increased with respect to the initial number of cells, revealing the cytocompatibility of the substrate. In addition, the differentiated cells on the PVA scaffolds expressed anti-troponin T, a marker specific of the cardiac sarcomere. We demonstrated the ability of the cardiomyocytes to pulse within the scaffolds. In conclusion, the developed scaffold show the potential to be used as a biomaterial for CTE applications.

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U2 - 10.1039/c8ra08187e

DO - 10.1039/c8ra08187e

M3 - Article

SN - 2046-2069

VL - 9

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EP - 4257

JO - RSC Advances

JF - RSC Advances

IS - 8

ER -

Development of 3D PVA scaffolds for cardiac tissue engineering and cell screening applications

Abstract

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