TY - JOUR
T1 - Development and validation of an automated high-throughput system for zebrafish in vivo screenings.
AU - Letamendia, Ainhoa
AU - Quevedo, Celia
AU - Ibarbia, Izaskun
AU - Virto, Juan M.
AU - Holgado, Olaia
AU - Diez, Maria
AU - Izpisua Belmonte, Juan Carlos
AU - Callol-Massot, Carles
N1 - Copyright:
This record is sourced from MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
PY - 2012
Y1 - 2012
N2 - The zebrafish is a vertebrate model compatible with the paradigms of drug discovery. The small size and transparency of zebrafish embryos make them amenable for the automation necessary in high-throughput screenings. We have developed an automated high-throughput platform for in vivo chemical screenings on zebrafish embryos that includes automated methods for embryo dispensation, compound delivery, incubation, imaging and analysis of the results. At present, two different assays to detect cardiotoxic compounds and angiogenesis inhibitors can be automatically run in the platform, showing the versatility of the system. A validation of these two assays with known positive and negative compounds, as well as a screening for the detection of unknown anti-angiogenic compounds, have been successfully carried out in the system developed. We present a totally automated platform that allows for high-throughput screenings in a vertebrate organism.
AB - The zebrafish is a vertebrate model compatible with the paradigms of drug discovery. The small size and transparency of zebrafish embryos make them amenable for the automation necessary in high-throughput screenings. We have developed an automated high-throughput platform for in vivo chemical screenings on zebrafish embryos that includes automated methods for embryo dispensation, compound delivery, incubation, imaging and analysis of the results. At present, two different assays to detect cardiotoxic compounds and angiogenesis inhibitors can be automatically run in the platform, showing the versatility of the system. A validation of these two assays with known positive and negative compounds, as well as a screening for the detection of unknown anti-angiogenic compounds, have been successfully carried out in the system developed. We present a totally automated platform that allows for high-throughput screenings in a vertebrate organism.
UR - http://www.scopus.com/inward/record.url?scp=84866178742&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0036690
DO - 10.1371/journal.pone.0036690
M3 - Article
C2 - 22615792
AN - SCOPUS:84866178742
SN - 1932-6203
VL - 7
SP - e36690
JO - PloS one
JF - PloS one
IS - 5
ER -