Detection of marker associated with CTC in colorectal cancer in mononuclear cells of patients with benign inflammatory intestinal diseases

Johanna Born, Alexander Hendricks, Charlotte Hauser, Jan Hendrik Egberts, Thomas Becker, Christian Röder, Susanne Sebens*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Colorectal carcinoma (CRC) belongs to the most common tumor entities in western coun-tries. Circulating tumor cells (CTC) in blood of CRC patients are a powerful prognostic and predictive biomarker. However, whether CTC-associated markers can also be used for early CRC detection and discrimination from benign diseases is not known. This study investigated the presence of CTC-associated markers CK20, PLS3, LAD1, and DEFA5 in blood of patients with benign inflammatory intestinal disease (IID) and their correlation with malignancy. The detection rate of CK20 and DEFA5 significantly differed between diseased patients and healthy controls. LAD1 and PLS3 were detected in all samples with clear differences in gene expression. DEFA5 expression was higher in CRC and IID patients compared to healthy donors, while CK20 and PLS3 were lower in CRC compared to IID patients or healthy controls. Overall, all CTC-associated markers were detectable in blood of IID patients, but not correlating with inflammation severity. Finally, PLS3 emerged as a suitable marker for differentiation between malignant and non-malignant intestinal diseases or healthy controls, however its suitability for early CRC detection needs to be further validated.

Original languageEnglish (US)
Article number47
JournalCancers
Volume14
Issue number1
DOIs
StatePublished - Jan 1 2022

Bibliographical note

Publisher Copyright:
© 2021 by the authors. Licensee MDPI, Basel, Switzerland.

Keywords

  • Chronic intestinal inflammation
  • Circulating tumor cells
  • Colorectal cancer
  • Keratin-20
  • Liquid biopsy

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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