Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation

Anne Cammas; Brenda Janice Sanchez; Xian Jin Lian; Virginie Dormoy-Raclet; Kate Van Der Giessen; Isabel López De Silanes; Jennifer Ma; Carol Wilusz; John Richardson; Myriam Gorospe; Stefania Millevoi; Matteo Giovarelli; Roberto Gherzi; Sergio Di Marco; Imed Eddine Gallouzi

doi:10.1038/ncomms5190

Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation

Anne Cammas, Brenda Janice Sanchez, Xian Jin Lian, Virginie Dormoy-Raclet, Kate Van Der Giessen, Isabel López De Silanes, Jennifer Ma, Carol Wilusz, John Richardson, Myriam Gorospe, Stefania Millevoi, Matteo Giovarelli, Roberto Gherzi, Sergio Di Marco, Imed Eddine Gallouzi

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

HuR promotes myogenesis by stabilizing the MyoD, myogenin and p21 mRNAs during the fusion of muscle cells to form myotubes. Here we show that HuR, via a novel mRNA destabilizing activity, promotes the early steps of myogenesis by reducing the expression of the cell cycle promoter nucleophosmin (NPM). Depletion of HuR stabilizes the NPM mRNA, increases NPM protein levels and inhibits myogenesis, while its overexpression elicits the opposite effects. NPM mRNA destabilization involves the association of HuR with the decay factor KSRP as well as the ribonuclease PARN and the exosome. The C terminus of HuR mediates the formation of the HuR-KSRP complex and is sufficient for maintaining a low level of the NPM mRNA as well as promoting the commitment of muscle cells to myogenesis. We therefore propose a model whereby the downregulation of the NPM mRNA, mediated by HuR, KSRP and its associated ribonucleases, is required for proper myogenesis. © 2014 Macmillan Publishers Limited. All rights reserved.

Original language	English (US)
Journal	Nature Communications
Volume	5
DOIs	https://doi.org/10.1038/ncomms5190
State	Published - Jun 27 2014
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2022-09-13

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Chemistry
General Physics and Astronomy

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Cammas, A., Sanchez, B. J., Lian, X. J., Dormoy-Raclet, V., Van Der Giessen, K., De Silanes, I. L., Ma, J., Wilusz, C., Richardson, J., Gorospe, M., Millevoi, S., Giovarelli, M., Gherzi, R., Di Marco, S., & Gallouzi, I. E. (2014). Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation. Nature Communications, 5. https://doi.org/10.1038/ncomms5190

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title = "Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation",

abstract = "HuR promotes myogenesis by stabilizing the MyoD, myogenin and p21 mRNAs during the fusion of muscle cells to form myotubes. Here we show that HuR, via a novel mRNA destabilizing activity, promotes the early steps of myogenesis by reducing the expression of the cell cycle promoter nucleophosmin (NPM). Depletion of HuR stabilizes the NPM mRNA, increases NPM protein levels and inhibits myogenesis, while its overexpression elicits the opposite effects. NPM mRNA destabilization involves the association of HuR with the decay factor KSRP as well as the ribonuclease PARN and the exosome. The C terminus of HuR mediates the formation of the HuR-KSRP complex and is sufficient for maintaining a low level of the NPM mRNA as well as promoting the commitment of muscle cells to myogenesis. We therefore propose a model whereby the downregulation of the NPM mRNA, mediated by HuR, KSRP and its associated ribonucleases, is required for proper myogenesis. {\textcopyright} 2014 Macmillan Publishers Limited. All rights reserved.",

author = "Anne Cammas and Sanchez, {Brenda Janice} and Lian, {Xian Jin} and Virginie Dormoy-Raclet and {Van Der Giessen}, Kate and {De Silanes}, {Isabel L{\'o}pez} and Jennifer Ma and Carol Wilusz and John Richardson and Myriam Gorospe and Stefania Millevoi and Matteo Giovarelli and Roberto Gherzi and {Di Marco}, Sergio and Gallouzi, {Imed Eddine}",

note = "Generated from Scopus record by KAUST IRTS on 2022-09-13",

year = "2014",

month = jun,

day = "27",

doi = "10.1038/ncomms5190",

language = "English (US)",

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Cammas, A, Sanchez, BJ, Lian, XJ, Dormoy-Raclet, V, Van Der Giessen, K, De Silanes, IL, Ma, J, Wilusz, C, Richardson, J, Gorospe, M, Millevoi, S, Giovarelli, M, Gherzi, R, Di Marco, S & Gallouzi, IE 2014, 'Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation', Nature Communications, vol. 5. https://doi.org/10.1038/ncomms5190

TY - JOUR

T1 - Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation

AU - Cammas, Anne

AU - Sanchez, Brenda Janice

AU - Lian, Xian Jin

AU - Dormoy-Raclet, Virginie

AU - Van Der Giessen, Kate

AU - De Silanes, Isabel López

AU - Ma, Jennifer

AU - Wilusz, Carol

AU - Richardson, John

AU - Gorospe, Myriam

AU - Millevoi, Stefania

AU - Giovarelli, Matteo

AU - Gherzi, Roberto

AU - Di Marco, Sergio

AU - Gallouzi, Imed Eddine

N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13

PY - 2014/6/27

Y1 - 2014/6/27

N2 - HuR promotes myogenesis by stabilizing the MyoD, myogenin and p21 mRNAs during the fusion of muscle cells to form myotubes. Here we show that HuR, via a novel mRNA destabilizing activity, promotes the early steps of myogenesis by reducing the expression of the cell cycle promoter nucleophosmin (NPM). Depletion of HuR stabilizes the NPM mRNA, increases NPM protein levels and inhibits myogenesis, while its overexpression elicits the opposite effects. NPM mRNA destabilization involves the association of HuR with the decay factor KSRP as well as the ribonuclease PARN and the exosome. The C terminus of HuR mediates the formation of the HuR-KSRP complex and is sufficient for maintaining a low level of the NPM mRNA as well as promoting the commitment of muscle cells to myogenesis. We therefore propose a model whereby the downregulation of the NPM mRNA, mediated by HuR, KSRP and its associated ribonucleases, is required for proper myogenesis. © 2014 Macmillan Publishers Limited. All rights reserved.

AB - HuR promotes myogenesis by stabilizing the MyoD, myogenin and p21 mRNAs during the fusion of muscle cells to form myotubes. Here we show that HuR, via a novel mRNA destabilizing activity, promotes the early steps of myogenesis by reducing the expression of the cell cycle promoter nucleophosmin (NPM). Depletion of HuR stabilizes the NPM mRNA, increases NPM protein levels and inhibits myogenesis, while its overexpression elicits the opposite effects. NPM mRNA destabilization involves the association of HuR with the decay factor KSRP as well as the ribonuclease PARN and the exosome. The C terminus of HuR mediates the formation of the HuR-KSRP complex and is sufficient for maintaining a low level of the NPM mRNA as well as promoting the commitment of muscle cells to myogenesis. We therefore propose a model whereby the downregulation of the NPM mRNA, mediated by HuR, KSRP and its associated ribonucleases, is required for proper myogenesis. © 2014 Macmillan Publishers Limited. All rights reserved.

UR - http://www.nature.com/articles/ncomms5190

UR - http://www.scopus.com/inward/record.url?scp=84903647947&partnerID=8YFLogxK

U2 - 10.1038/ncomms5190

DO - 10.1038/ncomms5190

M3 - Article

SN - 2041-1723

VL - 5

JO - Nature Communications

JF - Nature Communications

ER -

Destabilization of nucleophosmin mRNA by the HuR/KSRP complex is required for muscle fibre formation

Abstract

Bibliographical note

ASJC Scopus subject areas

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