Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging

Liangliang Liang; Wei Yan; Xian Qin; Xiao Peng; Han Feng; Yu Wang; Ziyu Zhu; Lingmei Liu; Yu Han; Qinghua Xu; Junle Qu; Xiaogang Liu

doi:10.1002/ange.201912404

Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging

Liangliang Liang, Wei Yan, Xian Qin, Xiao Peng, Han Feng, Yu Wang, Ziyu Zhu, Lingmei Liu, Yu Han, Qinghua Xu, Junle Qu, Xiaogang Liu

Research output: Contribution to journal › Article › peer-review

Abstract

Stimulated emission depletion (STED) microscopy enables ultrastructural imaging of biological samples with high spatiotemporal resolution. STED nanoprobes based on fluorescent organosilica nanohybrids featuring sub-2 nm size and near-unity quantum yield are presented. The spin–orbit coupling (SOC) of heavy-atom-rich organic fluorophores is mitigated through a silane-molecule-mediated condensation/ dehalogenation process, resulting in bright fluorescent organosilica nanohybrids with multiple emitters in one hybrid nanodot. When harnessed as STED nanoprobes, these fluorescent nanohybrids show intense photoluminescence, high biocompatibility, and long-term photostability. Taking advantage of the low-power excitation (0.5 mW), prolonged singlet-state lifetime, and negligible depletion-induced re-excitation, these STED nanohybrids present high depletion efficiency (> 96%), extremely low saturation intensity (0.54 mW, ca.0.188 MWcm@2), and ultra-high lateral resolution (ca. lem/28).

Original language	English (US)
Pages (from-to)	756-761
Number of pages	6
Journal	Angewandte Chemie
Volume	132
Issue number	2
DOIs	https://doi.org/10.1002/ange.201912404
State	Published - Nov 27 2019

Bibliographical note

KAUST Repository Item: Exported on 2021-04-19
Acknowledgements: This work is supported by the Singapore Ministry of Education (MOE2017-T2-2-110), Agency for Science, Technology and Research (A*STAR) (Grant NO. A1883c0011), National Research Foundation, Prime MinisterQs Office, Singapore under its Competitive Research Program (Award No. NRF-CRP15-2015-03) and under the NRF Investigatorship programme (Award No. NRF-NRFI05-2019-0003), the National Key R&D Program of China (2017YFA0700500), the National Natural Science Foundation of China (21771135, 21701119, 61705137, 81727804, 61975127, 31771584),the Science and Technology Project of Shenzhen(KQJSCX20180328093614762). The computational work for
this article was supported by resources of the High Performance Computing System at National University of Singapore.

ASJC Scopus subject areas

General Medicine

Access to Document

10.1002/ange.201912404

Cite this

@article{31d35613fe3a4b9c9ca4c36126ef8d9d,

title = "Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging",

abstract = "Stimulated emission depletion (STED) microscopy enables ultrastructural imaging of biological samples with high spatiotemporal resolution. STED nanoprobes based on fluorescent organosilica nanohybrids featuring sub-2 nm size and near-unity quantum yield are presented. The spin–orbit coupling (SOC) of heavy-atom-rich organic fluorophores is mitigated through a silane-molecule-mediated condensation/ dehalogenation process, resulting in bright fluorescent organosilica nanohybrids with multiple emitters in one hybrid nanodot. When harnessed as STED nanoprobes, these fluorescent nanohybrids show intense photoluminescence, high biocompatibility, and long-term photostability. Taking advantage of the low-power excitation (0.5 mW), prolonged singlet-state lifetime, and negligible depletion-induced re-excitation, these STED nanohybrids present high depletion efficiency (> 96%), extremely low saturation intensity (0.54 mW, ca.0.188 MWcm@2), and ultra-high lateral resolution (ca. lem/28).",

author = "Liangliang Liang and Wei Yan and Xian Qin and Xiao Peng and Han Feng and Yu Wang and Ziyu Zhu and Lingmei Liu and Yu Han and Qinghua Xu and Junle Qu and Xiaogang Liu",

note = "KAUST Repository Item: Exported on 2021-04-19 Acknowledgements: This work is supported by the Singapore Ministry of Education (MOE2017-T2-2-110), Agency for Science, Technology and Research (A*STAR) (Grant NO. A1883c0011), National Research Foundation, Prime MinisterQs Office, Singapore under its Competitive Research Program (Award No. NRF-CRP15-2015-03) and under the NRF Investigatorship programme (Award No. NRF-NRFI05-2019-0003), the National Key R&D Program of China (2017YFA0700500), the National Natural Science Foundation of China (21771135, 21701119, 61705137, 81727804, 61975127, 31771584),the Science and Technology Project of Shenzhen(KQJSCX20180328093614762). The computational work for this article was supported by resources of the High Performance Computing System at National University of Singapore.",

year = "2019",

month = nov,

day = "27",

doi = "10.1002/ange.201912404",

language = "English (US)",

volume = "132",

pages = "756--761",

journal = "Angewandte Chemie",

issn = "0044-8249",

publisher = "Wiley",

number = "2",

}

TY - JOUR

T1 - Designing Sub-2 nm Organosilica Nanohybrids for Far-Field Super-Resolution Imaging

AU - Liang, Liangliang

AU - Yan, Wei

AU - Qin, Xian

AU - Peng, Xiao

AU - Feng, Han

AU - Wang, Yu

AU - Zhu, Ziyu

AU - Liu, Lingmei

AU - Han, Yu

AU - Xu, Qinghua

AU - Qu, Junle

AU - Liu, Xiaogang

N1 - KAUST Repository Item: Exported on 2021-04-19 Acknowledgements: This work is supported by the Singapore Ministry of Education (MOE2017-T2-2-110), Agency for Science, Technology and Research (A*STAR) (Grant NO. A1883c0011), National Research Foundation, Prime MinisterQs Office, Singapore under its Competitive Research Program (Award No. NRF-CRP15-2015-03) and under the NRF Investigatorship programme (Award No. NRF-NRFI05-2019-0003), the National Key R&D Program of China (2017YFA0700500), the National Natural Science Foundation of China (21771135, 21701119, 61705137, 81727804, 61975127, 31771584),the Science and Technology Project of Shenzhen(KQJSCX20180328093614762). The computational work for this article was supported by resources of the High Performance Computing System at National University of Singapore.

PY - 2019/11/27

Y1 - 2019/11/27

N2 - Stimulated emission depletion (STED) microscopy enables ultrastructural imaging of biological samples with high spatiotemporal resolution. STED nanoprobes based on fluorescent organosilica nanohybrids featuring sub-2 nm size and near-unity quantum yield are presented. The spin–orbit coupling (SOC) of heavy-atom-rich organic fluorophores is mitigated through a silane-molecule-mediated condensation/ dehalogenation process, resulting in bright fluorescent organosilica nanohybrids with multiple emitters in one hybrid nanodot. When harnessed as STED nanoprobes, these fluorescent nanohybrids show intense photoluminescence, high biocompatibility, and long-term photostability. Taking advantage of the low-power excitation (0.5 mW), prolonged singlet-state lifetime, and negligible depletion-induced re-excitation, these STED nanohybrids present high depletion efficiency (> 96%), extremely low saturation intensity (0.54 mW, ca.0.188 MWcm@2), and ultra-high lateral resolution (ca. lem/28).

AB - Stimulated emission depletion (STED) microscopy enables ultrastructural imaging of biological samples with high spatiotemporal resolution. STED nanoprobes based on fluorescent organosilica nanohybrids featuring sub-2 nm size and near-unity quantum yield are presented. The spin–orbit coupling (SOC) of heavy-atom-rich organic fluorophores is mitigated through a silane-molecule-mediated condensation/ dehalogenation process, resulting in bright fluorescent organosilica nanohybrids with multiple emitters in one hybrid nanodot. When harnessed as STED nanoprobes, these fluorescent nanohybrids show intense photoluminescence, high biocompatibility, and long-term photostability. Taking advantage of the low-power excitation (0.5 mW), prolonged singlet-state lifetime, and negligible depletion-induced re-excitation, these STED nanohybrids present high depletion efficiency (> 96%), extremely low saturation intensity (0.54 mW, ca.0.188 MWcm@2), and ultra-high lateral resolution (ca. lem/28).

UR - http://hdl.handle.net/10754/668759

UR - https://onlinelibrary.wiley.com/doi/abs/10.1002/ange.201912404

U2 - 10.1002/ange.201912404

DO - 10.1002/ange.201912404

M3 - Article

SN - 0044-8249

VL - 132

SP - 756

EP - 761

JO - Angewandte Chemie

JF - Angewandte Chemie

IS - 2

ER -