Design, Simulation, and Evaluation of Polymer-Based Microfluidic Devices via Computational Fluid Dynamics and Cell Culture “On-Chip”

Nurzhanna Bakuova, Sultanali Toktarkan, Darkhan Dyussembinov, Dulat Azhibek, Almas Rakhymzhanov, Konstantinos Kostas, Gulsim Kulsharova

    Research output: Contribution to journalArticlepeer-review

    4 Scopus citations

    Abstract

    Organ-on-a-chip (OoC) technology has experienced exponential growth driven by the need for a better understanding of in-organ processes and the development of novel approaches. This paper investigates and compares the flow behavior and filling characteristics of two microfluidic liver-on-a-chip devices using Computational Fluid Dynamics (CFD) analysis and experimental cell culture growth based on the Huh7 cell line. The conducted computational analyses for the two chips showed that the elliptical chamber chip proposed herein offers improved flow and filling characteristics in comparison with the previously presented circular chamber chip. Huh7 hepatoma cells were cultured in the microfluidic devices for 24 h under static fluidic conditions and for 24 h with a flow rate of 3 μL·min−1. Biocompatibility, continuous flow, and biomarker studies showed cell attachment in the chips, confirming the cell viability and their consistent cell growth. The study successfully analyzed the fluid flow behavior, filling characteristics, and biocompatibility of liver-on-a-chip prototype devices, providing valuable insights to improve design and performance and advance alternative methods of in vitro testing.
    Original languageEnglish (US)
    Pages (from-to)754
    JournalBiosensors
    Volume13
    Issue number7
    DOIs
    StatePublished - Jul 22 2023

    Bibliographical note

    KAUST Repository Item: Exported on 2023-07-27
    Acknowledgements: This research was funded by the Ministry of Education and Science of the Republic of Kazakhstan Grant for young scientists (AP09058308) and Nazarbayev University FDCRG grant (080420FD1910). We thank Zhussipbek Mukhatayev for providing cells, Bereke Dauletkanov for guidance in chip fabrication, Akbota Kurmangaliyeva for assistance in the cell count, Luis Solorzano Rojas and Elvira Darbayeva for the contribution to the optimization of the device design.

    ASJC Scopus subject areas

    • Clinical Biochemistry

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