Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy; Maged M. Henary; Nisan Siegel; Pa Dreyia V. Lawson; Yonggang Wu; Karin Schmidt; Jean Luc Brédas; Joseph W. Perry; Christoph J. Fahrni

doi:10.1021/ja073240o

Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

S. Sumalekshmy, Maged M. Henary, Nisan Siegel, Pa Dreyia V. Lawson, Yonggang Wu, Karin Schmidt, Jean Luc Brédas^*, Joseph W. Perry, Christoph J. Fahrni

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

117 Scopus citations

Abstract

To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

Original language	English (US)
Pages (from-to)	11888-11889
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	129
Issue number	39
DOIs	https://doi.org/10.1021/ja073240o
State	Published - Oct 3 2007
Externally published	Yes

ASJC Scopus subject areas

Catalysis
General Chemistry
Biochemistry
Colloid and Surface Chemistry

Access to Document

10.1021/ja073240o

Cite this

@article{9e7bd5ceb8014e9ca176e0ca9aba38a1,

title = "Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness",

abstract = "To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.",

author = "S. Sumalekshmy and Henary, {Maged M.} and Nisan Siegel and Lawson, {Pa Dreyia V.} and Yonggang Wu and Karin Schmidt and Br{\'e}das, {Jean Luc} and Perry, {Joseph W.} and Fahrni, {Christoph J.}",

year = "2007",

month = oct,

day = "3",

doi = "10.1021/ja073240o",

language = "English (US)",

volume = "129",

pages = "11888--11889",

journal = "Journal of the American Chemical Society",

issn = "0002-7863",

publisher = "American Chemical Society",

number = "39",

}

TY - JOUR

T1 - Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

AU - Sumalekshmy, S.

AU - Henary, Maged M.

AU - Siegel, Nisan

AU - Lawson, Pa Dreyia V.

AU - Wu, Yonggang

AU - Schmidt, Karin

AU - Brédas, Jean Luc

AU - Perry, Joseph W.

AU - Fahrni, Christoph J.

PY - 2007/10/3

Y1 - 2007/10/3

N2 - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

AB - To develop metal-responsive fluorescence probes with optimized nonlinear optical properties for two-photon excitation fluorescence microscopy (TPEM), we investigated the utility of donor-acceptor architectures where the metal-ion coordinates to the acceptor rather than donor site of the fluorophore. Zn(II) binding to such a probe consisting of a tris(picolyl)amine-substituted acceptor site connected with an anisole donor moiety and an oxazole central core resulted in a significant increase of the two-photon absorption cross section (δ) and fluorescence brightness (ηδ). The fluorophore showed also a strong shift of the emission peak upon saturation with Zn(II), rendering the proposed approach suitable for two-photon excited emission ratiometric sensing.

UR - http://www.scopus.com/inward/record.url?scp=34848820596&partnerID=8YFLogxK

U2 - 10.1021/ja073240o

DO - 10.1021/ja073240o

M3 - Article

C2 - 17845038

AN - SCOPUS:34848820596

SN - 0002-7863

VL - 129

SP - 11888

EP - 11889

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

IS - 39

ER -

Design of emission ratiometric metal-ion sensors with enhanced two-photon cross section and brightness

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this