TY - JOUR
T1 - Depletion of HuR in murine skeletal muscle enhances exercise endurance and prevents cancer-induced muscle atrophy
AU - Janice Sánchez, Brenda
AU - Tremblay, Anne Marie K.
AU - Leduc-Gaudet, Jean Philippe
AU - Hall, Derek T.
AU - Kovacs, Erzsebet
AU - Ma, Jennifer F.
AU - Mubaid, Souad
AU - Hallauer, Patricia L.
AU - Phillips, Brittany L.
AU - Vest, Katherine E.
AU - Corbett, Anita H.
AU - Kontoyiannis, Dimitris L.
AU - Hussain, Sabah N.A.
AU - Hastings, Kenneth E.M.
AU - Di Marco, Sergio
AU - Gallouzi, Imed Eddine
N1 - Generated from Scopus record by KAUST IRTS on 2022-09-13
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The master posttranscriptional regulator HuR promotes muscle fiber formation in cultured muscle cells. However, its impact on muscle physiology and function in vivo is still unclear. Here, we show that muscle-specific HuR knockout (muHuR-KO) mice have high exercise endurance that is associated with enhanced oxygen consumption and carbon dioxide production. muHuR-KO mice exhibit a significant increase in the proportion of oxidative type I fibers in several skeletal muscles. HuR mediates these effects by collaborating with the mRNA decay factor KSRP to destabilize the PGC-1α mRNA. The type I fiber-enriched phenotype of muHuR-KO mice protects against cancer cachexia-induced muscle loss. Therefore, our study uncovers that under normal conditions HuR modulates muscle fiber type specification by promoting the formation of glycolytic type II fibers. We also provide a proof-of-principle that HuR expression can be targeted therapeutically in skeletal muscles to combat cancer-induced muscle wasting.
AB - The master posttranscriptional regulator HuR promotes muscle fiber formation in cultured muscle cells. However, its impact on muscle physiology and function in vivo is still unclear. Here, we show that muscle-specific HuR knockout (muHuR-KO) mice have high exercise endurance that is associated with enhanced oxygen consumption and carbon dioxide production. muHuR-KO mice exhibit a significant increase in the proportion of oxidative type I fibers in several skeletal muscles. HuR mediates these effects by collaborating with the mRNA decay factor KSRP to destabilize the PGC-1α mRNA. The type I fiber-enriched phenotype of muHuR-KO mice protects against cancer cachexia-induced muscle loss. Therefore, our study uncovers that under normal conditions HuR modulates muscle fiber type specification by promoting the formation of glycolytic type II fibers. We also provide a proof-of-principle that HuR expression can be targeted therapeutically in skeletal muscles to combat cancer-induced muscle wasting.
UR - http://www.nature.com/articles/s41467-019-12186-6
UR - http://www.scopus.com/inward/record.url?scp=85071399536&partnerID=8YFLogxK
U2 - 10.1038/s41467-019-12186-6
DO - 10.1038/s41467-019-12186-6
M3 - Article
SN - 2041-1723
VL - 10
JO - Nature Communications
JF - Nature Communications
IS - 1
ER -
