Decreasing hydraulic retention time of anaerobic membrane bioreactor: Effect on core genera and microbial contaminants removal

Julie Sanchez Medina; Shuo Zhang; Changzhi Wang; Jianqiang Zhou; Pei Ying Hong

doi:10.1016/j.biteb.2023.101389

Decreasing hydraulic retention time of anaerobic membrane bioreactor: Effect on core genera and microbial contaminants removal

Julie Sanchez Medina, Shuo Zhang, Changzhi Wang, Jianqiang Zhou, Pei Ying Hong^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A pilot-scale (35 L) anaerobic membrane bioreactor (AnMBR) treating municipal wastewater was operated under decreasing hydraulic retention times (HRT) of 12 h, 10 h and 8 h, and evaluated for its performance and removal of bacterial cells. There was an increased variability in COD and bacterial cells removal when HRT was 8 h, and a positive net growth rate of potential pathogen-containing genera Mycobacterium sp. was reported when AnMBR was operated at HRT of 8 h. Specifically, metagenomic assembled genomes of opportunistic pathogenic Mycobacterium franklinii were reconstructed uniquely in the 8 h HRT effluent. In contrast, co-occurrence network results showed when the HRT was 10 h, the microbial interactions were higher and bacterial syntrophs and methanogens had a more prevalent role in the microbial network. Our findings suggest that an optimal HRT (10 h) is required to achieve stable AnMBR performance and removal of microbial contaminants from untreated municipal wastewater, and can facilitate proper sizing of AnMBR.

Original language	English (US)
Article number	101389
Journal	Bioresource Technology Reports
Volume	22
DOIs	https://doi.org/10.1016/j.biteb.2023.101389
State	Published - Jun 2023

Bibliographical note

Funding Information:
This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling.

Funding Information:
This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling.

Publisher Copyright:
© 2023 The Authors

Keywords

Antimicrobial resistome
Metagenomics
Microbial co-occurrence networks
Municipal wastewater
Mycobacterium

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.biteb.2023.101389

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title = "Decreasing hydraulic retention time of anaerobic membrane bioreactor: Effect on core genera and microbial contaminants removal",

abstract = "A pilot-scale (35 L) anaerobic membrane bioreactor (AnMBR) treating municipal wastewater was operated under decreasing hydraulic retention times (HRT) of 12 h, 10 h and 8 h, and evaluated for its performance and removal of bacterial cells. There was an increased variability in COD and bacterial cells removal when HRT was 8 h, and a positive net growth rate of potential pathogen-containing genera Mycobacterium sp. was reported when AnMBR was operated at HRT of 8 h. Specifically, metagenomic assembled genomes of opportunistic pathogenic Mycobacterium franklinii were reconstructed uniquely in the 8 h HRT effluent. In contrast, co-occurrence network results showed when the HRT was 10 h, the microbial interactions were higher and bacterial syntrophs and methanogens had a more prevalent role in the microbial network. Our findings suggest that an optimal HRT (10 h) is required to achieve stable AnMBR performance and removal of microbial contaminants from untreated municipal wastewater, and can facilitate proper sizing of AnMBR.",

keywords = "Antimicrobial resistome, Metagenomics, Microbial co-occurrence networks, Municipal wastewater, Mycobacterium",

author = "Medina, {Julie Sanchez} and Shuo Zhang and Changzhi Wang and Jianqiang Zhou and Hong, {Pei Ying}",

note = "Funding Information: This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling. Funding Information: This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling. Publisher Copyright: {\textcopyright} 2023 The Authors",

year = "2023",

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doi = "10.1016/j.biteb.2023.101389",

language = "English (US)",

volume = "22",

journal = "Bioresource Technology Reports",

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AU - Medina, Julie Sanchez

AU - Zhang, Shuo

AU - Wang, Changzhi

AU - Zhou, Jianqiang

AU - Hong, Pei Ying

N1 - Funding Information: This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling. Funding Information: This study was supported by the KAUST WDRC CCF fund FCC/1/1941-43-01 awarded Peiying Hong. The authors would like to thank the KAUST FM Utilities team for their assistance in sampling. Publisher Copyright: © 2023 The Authors

PY - 2023/6

Y1 - 2023/6

N2 - A pilot-scale (35 L) anaerobic membrane bioreactor (AnMBR) treating municipal wastewater was operated under decreasing hydraulic retention times (HRT) of 12 h, 10 h and 8 h, and evaluated for its performance and removal of bacterial cells. There was an increased variability in COD and bacterial cells removal when HRT was 8 h, and a positive net growth rate of potential pathogen-containing genera Mycobacterium sp. was reported when AnMBR was operated at HRT of 8 h. Specifically, metagenomic assembled genomes of opportunistic pathogenic Mycobacterium franklinii were reconstructed uniquely in the 8 h HRT effluent. In contrast, co-occurrence network results showed when the HRT was 10 h, the microbial interactions were higher and bacterial syntrophs and methanogens had a more prevalent role in the microbial network. Our findings suggest that an optimal HRT (10 h) is required to achieve stable AnMBR performance and removal of microbial contaminants from untreated municipal wastewater, and can facilitate proper sizing of AnMBR.

AB - A pilot-scale (35 L) anaerobic membrane bioreactor (AnMBR) treating municipal wastewater was operated under decreasing hydraulic retention times (HRT) of 12 h, 10 h and 8 h, and evaluated for its performance and removal of bacterial cells. There was an increased variability in COD and bacterial cells removal when HRT was 8 h, and a positive net growth rate of potential pathogen-containing genera Mycobacterium sp. was reported when AnMBR was operated at HRT of 8 h. Specifically, metagenomic assembled genomes of opportunistic pathogenic Mycobacterium franklinii were reconstructed uniquely in the 8 h HRT effluent. In contrast, co-occurrence network results showed when the HRT was 10 h, the microbial interactions were higher and bacterial syntrophs and methanogens had a more prevalent role in the microbial network. Our findings suggest that an optimal HRT (10 h) is required to achieve stable AnMBR performance and removal of microbial contaminants from untreated municipal wastewater, and can facilitate proper sizing of AnMBR.

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KW - Metagenomics

KW - Microbial co-occurrence networks

KW - Municipal wastewater

KW - Mycobacterium

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Decreasing hydraulic retention time of anaerobic membrane bioreactor: Effect on core genera and microbial contaminants removal

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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