Functional genomics is at the core of studying the exact function of genes. However, homozygous knockouts of essential and pleiotropic effectors (almost 10% of the genome) are not always possible, thus, functions of these genes remain obscured. The tissue-specific genome editing tool (CRISPR-TSKO) recently described by Decaestecker et al., can characterize these indispensable genes and has wide applications in plants.
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work in the lab of S.M. is supported by a research grant from Higher Education Commission, Government of Pakistan.