CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu, Marcela Vilarino, Keiichiro Suzuki, Daiji Okamura, Yanina Soledad Bogliotti, Insung Park, Joan Rowe, Bret McNabb, Pablo Juan Ross*, Juan Carlos Izpisua Belmonte

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

28 Scopus citations


Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

Original languageEnglish (US)
Article number10487
JournalScientific Reports
Issue number1
StatePublished - Dec 1 2017

Bibliographical note

Funding Information:
We thank Aaron Prinz, Kent Parker, and their crews for excellent assistance with embryo transfers and pig care at UC Davis. We would like to thank M. Schwarz, and P. Schwarz for administrative help. P.J.R. was supported by UC Davis Academic Senate New Research Grant. Work in the laboratory of JCIB was supported by the UCAM, G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation.

Publisher Copyright:
© 2017 The Author(s).

ASJC Scopus subject areas

  • General


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