CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu; Marcela Vilarino; Keiichiro Suzuki; Daiji Okamura; Yanina Soledad Bogliotti; Insung Park; Joan Rowe; Bret McNabb; Pablo Juan Ross; Juan Carlos Izpisua Belmonte

doi:10.1038/s41598-017-08596-5

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu, Marcela Vilarino, Keiichiro Suzuki, Daiji Okamura, Yanina Soledad Bogliotti, Insung Park, Joan Rowe, Bret McNabb, Pablo Juan Ross^*, Juan Carlos Izpisua Belmonte

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

Original language	English (US)
Article number	10487
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-08596-5
State	Published - Dec 1 2017

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© 2017 The Author(s).

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abstract = "Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.",

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AU - Vilarino, Marcela

AU - Suzuki, Keiichiro

AU - Okamura, Daiji

AU - Bogliotti, Yanina Soledad

AU - Park, Insung

AU - Rowe, Joan

AU - McNabb, Bret

AU - Ross, Pablo Juan

AU - Belmonte, Juan Carlos Izpisua

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AB - Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

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CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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