CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu; Marcela Vilarino; Keiichiro Suzuki; Daiji Okamura; Yanina Soledad Bogliotti; Insung Park; Joan Rowe; Bret McNabb; Pablo Juan Ross; Juan Carlos Izpisua Belmonte

doi:10.1038/s41598-017-08596-5

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Jun Wu, Marcela Vilarino, Keiichiro Suzuki, Daiji Okamura, Yanina Soledad Bogliotti, Insung Park, Joan Rowe, Bret McNabb, Pablo Juan Ross^*, Juan Carlos Izpisua Belmonte

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

Original language	English (US)
Article number	10487
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-08596-5
State	Published - Dec 1 2017

Bibliographical note

Funding Information:
We thank Aaron Prinz, Kent Parker, and their crews for excellent assistance with embryo transfers and pig care at UC Davis. We would like to thank M. Schwarz, and P. Schwarz for administrative help. P.J.R. was supported by UC Davis Academic Senate New Research Grant. Work in the laboratory of JCIB was supported by the UCAM, G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation.

Publisher Copyright:
© 2017 The Author(s).

ASJC Scopus subject areas

General

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41598-017-08596-5

Cite this

@article{ca930f69db5a4e6f98f8db26c48cebca,

title = "CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs",

abstract = "Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.",

author = "Jun Wu and Marcela Vilarino and Keiichiro Suzuki and Daiji Okamura and Bogliotti, {Yanina Soledad} and Insung Park and Joan Rowe and Bret McNabb and Ross, {Pablo Juan} and Belmonte, {Juan Carlos Izpisua}",

note = "Funding Information: We thank Aaron Prinz, Kent Parker, and their crews for excellent assistance with embryo transfers and pig care at UC Davis. We would like to thank M. Schwarz, and P. Schwarz for administrative help. P.J.R. was supported by UC Davis Academic Senate New Research Grant. Work in the laboratory of JCIB was supported by the UCAM, G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation. Publisher Copyright: {\textcopyright} 2017 The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-08596-5",

language = "English (US)",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Publishing Group",

number = "1",

}

TY - JOUR

T1 - CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

AU - Wu, Jun

AU - Vilarino, Marcela

AU - Suzuki, Keiichiro

AU - Okamura, Daiji

AU - Bogliotti, Yanina Soledad

AU - Park, Insung

AU - Rowe, Joan

AU - McNabb, Bret

AU - Ross, Pablo Juan

AU - Belmonte, Juan Carlos Izpisua

N1 - Funding Information: We thank Aaron Prinz, Kent Parker, and their crews for excellent assistance with embryo transfers and pig care at UC Davis. We would like to thank M. Schwarz, and P. Schwarz for administrative help. P.J.R. was supported by UC Davis Academic Senate New Research Grant. Work in the laboratory of JCIB was supported by the UCAM, G. Harold and Leila Y. Mathers Charitable Foundation and The Moxie Foundation. Publisher Copyright: © 2017 The Author(s).

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

AB - Genome editing using programmable nucleases has revolutionized biomedical research. CRISPR-Cas9 mediated zygote genome editing enables high efficient production of knockout animals suitable for studying development and relevant human diseases. Here we report efficient disabling pancreatogenesis in pig embryos via zygotic co-delivery of Cas9 mRNA and dual sgRNAs targeting the PDX1 gene, which when combined with chimeric-competent human pluriopotent stem cells may serve as a suitable platform for the xeno-generation of human tissues and organs in pigs.

UR - http://www.scopus.com/inward/record.url?scp=85028880250&partnerID=8YFLogxK

U2 - 10.1038/s41598-017-08596-5

DO - 10.1038/s41598-017-08596-5

M3 - Article

C2 - 28874671

AN - SCOPUS:85028880250

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 10487

ER -

CRISPR-Cas9 mediated one-step disabling of pancreatogenesis in pigs

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this