TY - JOUR
T1 - CRISPR-Based Directed Evolution for Crop Improvement.
AU - Butt, Haroon
AU - Zaidi, Syed Shan-e-Ali
AU - Hassan, Norhan
AU - Mahfouz, Magdy M.
N1 - KAUST Repository Item: Exported on 2020-10-01
PY - 2019/8/30
Y1 - 2019/8/30
N2 - Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.
AB - Directed evolution involves generating diverse sequence variants of a gene of interest to produce a desirable trait under selective pressure. CRISPR-Cas9 (clustered regularly interspaced short palindromic repeats-CRISPR-associated protein 9) systems can be programmed to target any genomic locus and perform targeted directed evolution. Here, we discuss the opportunities and challenges of this emerging platform for targeted crop improvement.
UR - http://hdl.handle.net/10754/656702
UR - https://linkinghub.elsevier.com/retrieve/pii/S0167779919302057
UR - http://www.scopus.com/inward/record.url?scp=85071485513&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2019.08.001
DO - 10.1016/j.tibtech.2019.08.001
M3 - Article
C2 - 31477243
SN - 0167-7799
JO - Trends in biotechnology
JF - Trends in biotechnology
ER -