Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis

Shakeel Ahmed Ansari; Rukhsana Satar; Fahad Alam; Mohammed Husein Alqahtani; Adeel Gulzar Chaudhary; Muhammad Imran Naseer; Sajjad Karim; Ishfaq Ahmed Sheikh

doi:10.1016/j.procbio.2012.10.002

Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis

Shakeel Ahmed Ansari, Rukhsana Satar, Fahad Alam, Mohammed Husein Alqahtani, Adeel Gulzar Chaudhary, Muhammad Imran Naseer, Sajjad Karim, Ishfaq Ahmed Sheikh

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.

Original language	English (US)
Pages (from-to)	2427-2433
Number of pages	7
Journal	Process Biochemistry
Volume	47
Issue number	12
DOIs	https://doi.org/10.1016/j.procbio.2012.10.002
State	Published - Dec 1 2012
Externally published	Yes

Bibliographical note

Generated from Scopus record by KAUST IRTS on 2023-09-23

ASJC Scopus subject areas

Biochemistry
Organic Chemistry
Engineering (miscellaneous)
Industrial and Manufacturing Engineering

Access to Document

10.1016/j.procbio.2012.10.002

Cite this

Ansari, S. A., Satar, R., Alam, F., Alqahtani, M. H., Chaudhary, A. G., Naseer, M. I., Karim, S., & Sheikh, I. A. (2012). Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis. Process Biochemistry, 47(12), 2427-2433. https://doi.org/10.1016/j.procbio.2012.10.002

@article{63d10aeba7924be7b2762cbbf95eb93e,

title = "Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis",

abstract = "The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright {\textcopyright} 2012 Published by Elsevier Ltd. All rights reserved.",

author = "Ansari, {Shakeel Ahmed} and Rukhsana Satar and Fahad Alam and Alqahtani, {Mohammed Husein} and Chaudhary, {Adeel Gulzar} and Naseer, {Muhammad Imran} and Sajjad Karim and Sheikh, {Ishfaq Ahmed}",

note = "Generated from Scopus record by KAUST IRTS on 2023-09-23",

year = "2012",

month = dec,

day = "1",

doi = "10.1016/j.procbio.2012.10.002",

language = "English (US)",

volume = "47",

pages = "2427--2433",

journal = "Process Biochemistry",

issn = "1359-5113",

publisher = "Elsevier BV",

number = "12",

}

Ansari, SA, Satar, R, Alam, F, Alqahtani, MH, Chaudhary, AG, Naseer, MI, Karim, S & Sheikh, IA 2012, 'Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis', Process Biochemistry, vol. 47, no. 12, pp. 2427-2433. https://doi.org/10.1016/j.procbio.2012.10.002

Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis. / Ansari, Shakeel Ahmed; Satar, Rukhsana; Alam, Fahad et al.
In: Process Biochemistry, Vol. 47, No. 12, 01.12.2012, p. 2427-2433.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis

AU - Ansari, Shakeel Ahmed

AU - Satar, Rukhsana

AU - Alam, Fahad

AU - Alqahtani, Mohammed Husein

AU - Chaudhary, Adeel Gulzar

AU - Naseer, Muhammad Imran

AU - Karim, Sajjad

AU - Sheikh, Ishfaq Ahmed

N1 - Generated from Scopus record by KAUST IRTS on 2023-09-23

PY - 2012/12/1

Y1 - 2012/12/1

N2 - The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.

AB - The present study demonstrates synthesis, characterization and surface functionalization of silver nanoparticles (AgNPs) via glutaraldehyde for high yield immobilization of Aspergillus oryzae β-galactosidase. Soluble β-galactosidase (SβG), enzyme adsorbed on unmodified AgNPs (UβG) and surface modified AgNPs (MβG) showed same pH-optima at pH 4.5. However, it was observed that MβG exhibited enhanced pH stability toward acidic and alkaline sides, and increased temperature resistance as compared to SβG and UβG. Michaelis constant, Km was increased nearly three-folds for MβG while Vmax for soluble and MβG was 0.515 mM/min and 0.495 mM/min, respectively. Furthermore, MβG showed greater resistance to product inhibition mediated by galactose as compared to it soluble counterpart and exhibited excellent catalytic activity even after its fourth successive reuse. The remarkable bioconversion rates of lactose from milk in batch reactors further revealed an attractive catalytic efficiency of β-galactosidase adsorbed on surface functionalized AgNPs thereby promoting its use in the production of lactose free dairy products. Crown Copyright © 2012 Published by Elsevier Ltd. All rights reserved.

UR - https://linkinghub.elsevier.com/retrieve/pii/S1359511312003716

UR - http://www.scopus.com/inward/record.url?scp=84870840125&partnerID=8YFLogxK

U2 - 10.1016/j.procbio.2012.10.002

DO - 10.1016/j.procbio.2012.10.002

M3 - Article

SN - 1359-5113

VL - 47

SP - 2427

EP - 2433

JO - Process Biochemistry

JF - Process Biochemistry

IS - 12

ER -

Cost effective surface functionalization of silver nanoparticles for high yield immobilization of Aspergillus oryzae β-galactosidase and its application in lactose hydrolysis

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this