Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells

Francesca Pagliari; Elisa Sogne; Davide Panella; Gerardo Perozziello; Carlo Liberale; Gobind Das; Alice Turdo; Simone Di Franco; Joao Seco; Andrea Falqui; Santo Gratteri; Arturo Pujia; Enzo Di Fabrizio; Patrizio Candeloro; Luca Tirinato

doi:10.3390/bios12121102

Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells

Francesca Pagliari, Elisa Sogne, Davide Panella, Gerardo Perozziello, Carlo Liberale, Gobind Das, Alice Turdo, Simone Di Franco, Joao Seco, Andrea Falqui, Santo Gratteri, Arturo Pujia, Enzo Di Fabrizio, Patrizio Candeloro, Luca Tirinato

Research output: Contribution to journal › Article › peer-review

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Abstract

Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).

Original language	English (US)
Pages (from-to)	1102
Journal	Biosensors
Volume	12
Issue number	12
DOIs	https://doi.org/10.3390/bios12121102
State	Published - Dec 1 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-12-06
Acknowledgements: L.T. received funding from AIRC and the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement N° 800924. We are grateful to Federico Mecarini for his precious support and advice during SEM measurement.

ASJC Scopus subject areas

Clinical Biochemistry

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.3390/bios12121102

https://repository.kaust.edu.sa/bitstream/10754/686176/1/biosensors-12-01102.pdf

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Pagliari, F., Sogne, E., Panella, D., Perozziello, G., Liberale, C., Das, G., Turdo, A., Di Franco, S., Seco, J., Falqui, A., Gratteri, S., Pujia, A., Di Fabrizio, E., Candeloro, P., & Tirinato, L. (2022). Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells. Biosensors, 12(12), 1102. https://doi.org/10.3390/bios12121102

@article{dbda4606bd604ef2948064ff98182a68,

title = "Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells",

abstract = "Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).",

author = "Francesca Pagliari and Elisa Sogne and Davide Panella and Gerardo Perozziello and Carlo Liberale and Gobind Das and Alice Turdo and {Di Franco}, Simone and Joao Seco and Andrea Falqui and Santo Gratteri and Arturo Pujia and {Di Fabrizio}, Enzo and Patrizio Candeloro and Luca Tirinato",

note = "KAUST Repository Item: Exported on 2022-12-06 Acknowledgements: L.T. received funding from AIRC and the European Union{\textquoteright}s Horizon 2020 Research and Innovation Programme under the Marie Sk{\l}odowska-Curie grant agreement N° 800924. We are grateful to Federico Mecarini for his precious support and advice during SEM measurement.",

year = "2022",

month = dec,

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doi = "10.3390/bios12121102",

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Pagliari, F, Sogne, E, Panella, D, Perozziello, G, Liberale, C, Das, G, Turdo, A, Di Franco, S, Seco, J, Falqui, A, Gratteri, S, Pujia, A, Di Fabrizio, E, Candeloro, P & Tirinato, L 2022, 'Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells', Biosensors, vol. 12, no. 12, pp. 1102. https://doi.org/10.3390/bios12121102

TY - JOUR

T1 - Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells

AU - Pagliari, Francesca

AU - Sogne, Elisa

AU - Panella, Davide

AU - Perozziello, Gerardo

AU - Liberale, Carlo

AU - Das, Gobind

AU - Turdo, Alice

AU - Di Franco, Simone

AU - Seco, Joao

AU - Falqui, Andrea

AU - Gratteri, Santo

AU - Pujia, Arturo

AU - Di Fabrizio, Enzo

AU - Candeloro, Patrizio

AU - Tirinato, Luca

N1 - KAUST Repository Item: Exported on 2022-12-06 Acknowledgements: L.T. received funding from AIRC and the European Union’s Horizon 2020 Research and Innovation Programme under the Marie Skłodowska-Curie grant agreement N° 800924. We are grateful to Federico Mecarini for his precious support and advice during SEM measurement.

PY - 2022/12/1

Y1 - 2022/12/1

N2 - Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).

AB - Among all neoplasms, melanoma is characterized by a very high percentage of cancer stem cells (CSCs). Several markers have been proposed for their identification, and lipid droplets (LDs) are among them. Different techniques are used for their characterization such as mass spectrometry, imaging techniques, and vibrational spectroscopies. Some emerging experimental approaches for the study of LDs are represented by correlative light–electron microscopy and by correlative Raman imaging–scanning electron microscopy (SEM). Based on these scientific approaches, we developed a novel methodology (CREL) by combining Raman micro-spectroscopy, confocal fluorescence microscopy, and SEM coupled with an energy-dispersive X-ray spectroscopy module. This procedure correlated cellular morphology, chemical properties, and spatial distribution from the same region of interest, and in this work, we presented the application of CREL for the analysis of LDs within patient-derived melanoma CSCs (MCSCs).

UR - http://hdl.handle.net/10754/686176

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VL - 12

SP - 1102

JO - Biosensors

JF - Biosensors

IS - 12

ER -

Correlative Raman–Electron–Light (CREL) Microscopy Analysis of Lipid Droplets in Melanoma Cancer Stem Cells

Abstract

Bibliographical note

ASJC Scopus subject areas

UN SDGs

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