Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

N. Ideris; A. L. Ahmad; B. S. Ooi; S. C. Low

doi:10.1088/1757-899X/358/1/012054

Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

N. Ideris, A. L. Ahmad, B. S. Ooi, S. C. Low

Electrical and Computer Engineering

Research output: Contribution to conference › Paper › peer-review

1 Scopus citations

Abstract

Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R², of 0.9971.

Original language	English (US)
DOIs	https://doi.org/10.1088/1757-899X/358/1/012054
State	Published - May 22 2018
Event	3rd International Conference on Global Sustainability and Chemical Engineering, ICGSCE 2017 - Putrajaya, Malaysia Duration: Feb 15 2017 → Feb 16 2017

Conference

Conference	3rd International Conference on Global Sustainability and Chemical Engineering, ICGSCE 2017
Country/Territory	Malaysia
City	Putrajaya
Period	02/15/17 → 02/16/17

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Publisher Copyright:
© Published under licence by IOP Publishing Ltd.

ASJC Scopus subject areas

General Materials Science
General Engineering

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10.1088/1757-899X/358/1/012054

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title = "Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique",

abstract = "Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot{\textregistered} was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.",

author = "N. Ideris and Ahmad, {A. L.} and Ooi, {B. S.} and Low, {S. C.}",

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T1 - Correlation Study of PVDF Membrane Morphology with Protein Adsorption

T2 - 3rd International Conference on Global Sustainability and Chemical Engineering, ICGSCE 2017

AU - Ideris, N.

AU - Ahmad, A. L.

AU - Ooi, B. S.

AU - Low, S. C.

PY - 2018/5/22

Y1 - 2018/5/22

N2 - Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.

AB - Microporous PVDF membranes were used as protein capture matrices in immunoassays. Because the most common labels in immunoassays were detected based on the colour change, an understanding of how protein concentration varies on different PVDF surfaces was needed. Herein, the correlation between the membrane pore size and protein adsorption was systematically investigated. Five different PVDF membrane morphologies were prepared and FTIR/ATR was employed to accurately quantify the surface protein concentration on membranes with small pore sizes. SigmaPlot® was used to find a suitable curve fit for protein adsorption and membrane pore size, with a high correlation coefficient, R2, of 0.9971.

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DO - 10.1088/1757-899X/358/1/012054

M3 - Paper

AN - SCOPUS:85050609948

Y2 - 15 February 2017 through 16 February 2017

ER -

Correlation Study of PVDF Membrane Morphology with Protein Adsorption: Quantitative Analysis by FTIR/ATR Technique

Abstract

Conference

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