Continuous extraction and concentration of secreted metabolites from engineered microbes using membrane technology

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13 Scopus citations


Microalgal cultivation in photobioreactors and membrane separations are both considered sustainable processes. Here we explore their synergistic combination to extract and concentrate a heterologous sesquiterpenoid produced by engineered green algal cells. A hydrophobic hollow-fiber membrane contactor was used to allow interaction of culture broth and cells with a dodecane solvent phase to accumulate algal produced patchoulol. Subsequent continuous membrane extraction of patchoulol from dodecane enabled product concentration in a methanol stream as well as dodecane recovery for its reuse. A structure-based prediction using machine learning was employed to model a process whereby 100% patchoulol recovery from dodecane could be achieved with solvent-resistant nanofiltration membranes. Solvent consumption, E-factor, and economic sustainability were assessed and compared with existing patchoulol production processes. Our extraction and product purification process offers six- and two-orders of magnitude lower solvent consumption compared to synthetic production and thermal-based separation, respectively. Our proposed methodology is transferable to other microbial systems for the isolation of high-value isoprenoid and hydrocarbon products.

Original languageEnglish (US)
Pages (from-to)5479-5489
Number of pages11
JournalGreen Chemistry
Issue number14
StatePublished - May 18 2022

Bibliographical note

Funding Information:
We would like to express special thanks to Dr Najeh Kharbatia of the KAUST Analytical Core Labs for helpful early discussions, Chandrasekaren Lakshmipathy, Abdulkhalik Khalifa, and Abdullah Alabdullatif of KAUST Lab Equipment Maintenance (LEM) team for assistance in upgrading and initializing the GC-FID-MS unit. The authors acknowledge Prof. Dr Ralph Bock for providing C. reinhardtii UVM4, obtained under material transfer agreement between KAUST and the Max-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam. The research reported in this publication was supported by the KAUST Impact Acceleration Funds program (grant 4238), and KAUST baseline funding awarded to KL and GS. Fig. 1 was produced by Ana Bigio, scientific illustrator.

Publisher Copyright:
© 2022 The Royal Society of Chemistry.

ASJC Scopus subject areas

  • Environmental Chemistry
  • Pollution


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