Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

Yang-Yang Hou; Jing Xu; Fu-Ting Wang; Zhong Dong; Xuecai Tan; Ke-Jing Huang; Jia-Qiang Li; Chun-Yang Zuo; Si-Qi Zhang

doi:10.1021/acs.analchem.1c03521

Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

Yang-Yang Hou, Jing Xu, Fu-Ting Wang, Zhong Dong, Xuecai Tan, Ke-Jing Huang, Jia-Qiang Li, Chun-Yang Zuo, Si-Qi Zhang

King Abdullah University of Science and Technology

Research output: Contribution to journal › Article › peer-review

118 Scopus citations

Abstract

The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1–100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.

Original language	English (US)
Journal	Analytical Chemistry
DOIs	https://doi.org/10.1021/acs.analchem.1c03521
State	Published - Nov 9 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-11-11
Acknowledgements: The authors acknowledge financial support from the Natural Science Foundation of China (22074130, 21365004) and Zhongyuan Thousand Talents Plan-Science and Technology Innovation Leading Talents Project (204200510030). The authors acknowledge the support from the Analysis Testing Center of Xinyang Normal University for the materials characterization.

ASJC Scopus subject areas

Analytical Chemistry

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10.1021/acs.analchem.1c03521

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@article{7d09777df86d408c8b484b7c7fc9db71,

title = "Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection",

abstract = "The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1–100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.",

author = "Yang-Yang Hou and Jing Xu and Fu-Ting Wang and Zhong Dong and Xuecai Tan and Ke-Jing Huang and Jia-Qiang Li and Chun-Yang Zuo and Si-Qi Zhang",

note = "KAUST Repository Item: Exported on 2021-11-11 Acknowledgements: The authors acknowledge financial support from the Natural Science Foundation of China (22074130, 21365004) and Zhongyuan Thousand Talents Plan-Science and Technology Innovation Leading Talents Project (204200510030). The authors acknowledge the support from the Analysis Testing Center of Xinyang Normal University for the materials characterization.",

year = "2021",

month = nov,

day = "9",

doi = "10.1021/acs.analchem.1c03521",

language = "English (US)",

journal = "Analytical Chemistry",

issn = "0003-2700",

publisher = "American Chemical Society",

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TY - JOUR

T1 - Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

AU - Hou, Yang-Yang

AU - Xu, Jing

AU - Wang, Fu-Ting

AU - Dong, Zhong

AU - Tan, Xuecai

AU - Huang, Ke-Jing

AU - Li, Jia-Qiang

AU - Zuo, Chun-Yang

AU - Zhang, Si-Qi

N1 - KAUST Repository Item: Exported on 2021-11-11 Acknowledgements: The authors acknowledge financial support from the Natural Science Foundation of China (22074130, 21365004) and Zhongyuan Thousand Talents Plan-Science and Technology Innovation Leading Talents Project (204200510030). The authors acknowledge the support from the Analysis Testing Center of Xinyang Normal University for the materials characterization.

PY - 2021/11/9

Y1 - 2021/11/9

N2 - The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1–100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.

AB - The detection of microRNA (miRNA) in human serum has great significance for cancer prevention. Herein, a novel self-powered biosensing platform is developed, which effectively integrates an enzymatic biofuel cell (EBFC)-based self-powered biosensor with a matching capacitor for miRNA detection. A catalytic hairpin assembly and hybrid chain reaction are used to improve the analytical performance of EBFC. Furthermore, the matching capacitor is selected as an auxiliary signal amplifying device, and graphdiyne is applied as substrate material for EBFC. The results confirm that the developed method obviously increases the output current of EBFC, and the sensitivity can reach 2.75 μA/pM, which is 786% of pure EBFC. MiRNA can be detected in an expanded linear range of 0.1–100000 fM with a detection limit of 0.034 fM (S/N = 3). It can offer a selective and sensitive platform for nucleotide sequence detection with great potential in clinical diagnostics.

UR - http://hdl.handle.net/10754/673278

UR - https://pubs.acs.org/doi/10.1021/acs.analchem.1c03521

U2 - 10.1021/acs.analchem.1c03521

DO - 10.1021/acs.analchem.1c03521

M3 - Article

C2 - 34752059

SN - 0003-2700

JO - Analytical Chemistry

JF - Analytical Chemistry

ER -

Construction of an Integrated Device of a Self-Powered Biosensor and Matching Capacitor Based on Graphdiyne and Multiple Signal Amplification: Ultrasensitive Method for MicroRNA Detection

Abstract

Bibliographical note

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UN SDGs

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