Construction of a SNP and SSR linkage map in autotetraploid blueberry using genotyping by sequencing

Susan McCallum, Julie Graham, Linzi Jorgensen, Lisa J. Rowland, Nahla V. Bassil, James F. Hancock, Edmund J. Wheeler, Kelly Vining, Jesse A. Poland, James W. Olmstead, Emily Buck, Claudia Wiedow, Eric Jackson, Allan Brown, Christine A. Hackett

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The construction of the first genetic map in autotetraploid blueberry has been made possible by the development of new SNP markers developed using genotyping by sequencing in a mapping population created from a cross between two key highbush blueberry cultivars, Draper × Jewel (Vaccinium corymbosum). The novel SNP markers were supplemented with existing SSR markers to enable the alignment of parental maps.  In total, 1794 single nucleotide polymorphic (SNP) markers and 233 simple sequence repeat (SSR) markers exhibited segregation patterns consistent with a random chromosomal segregation model for meiosis in an autotetraploid. Of these, 700 SNPs and 85 SSRs were utilized for construction of the ‘Draper’ genetic map, and 450 SNPs and 86 SSRs for the ‘Jewel’ map.  The ‘Draper’ map comprises 12  linkage groups (LG), associated with the haploid chromosome number for blueberry, and totals 1621 cM while the ‘Jewel’ map comprises 20 linkage groups totalling 1610 cM. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents. Tentative alignments of the two parental maps have been made on the basis of shared SSR alleles and linkages to double-simplex markers segregating in both parents.
Original languageEnglish (US)
JournalMolecular Breeding
Volume36
Issue number4
DOIs
StatePublished - Apr 1 2016
Externally publishedYes

ASJC Scopus subject areas

  • Plant Science
  • Genetics
  • Molecular Biology
  • Agronomy and Crop Science
  • Biotechnology

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