Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi; Robert A. Creelman; John E. Mullet; Rod A. Wing

doi:10.1007/BF02668782

Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A. Wing

Research output: Contribution to journal › Article › peer-review

79 Scopus citations

Abstract

We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library. © 1995 Kluwer Academic Publishers.

Original language	English (US)
Journal	Plant Molecular Biology Reporter
Volume	13
Issue number	2
DOIs	https://doi.org/10.1007/BF02668782
State	Published - Jun 1 1995
Externally published	Yes

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title = "Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana",

abstract = "We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library. {\textcopyright} 1995 Kluwer Academic Publishers.",

author = "Sangdun Choi and Creelman, {Robert A.} and Mullet, {John E.} and Wing, {Rod A.}",

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year = "1995",

month = jun,

day = "1",

doi = "10.1007/BF02668782",

language = "English (US)",

volume = "13",

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T1 - Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

AU - Choi, Sangdun

AU - Creelman, Robert A.

AU - Mullet, John E.

AU - Wing, Rod A.

N1 - Generated from Scopus record by KAUST IRTS on 2019-11-20

PY - 1995/6/1

Y1 - 1995/6/1

N2 - We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library. © 1995 Kluwer Academic Publishers.

AB - We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library. © 1995 Kluwer Academic Publishers.

UR - http://link.springer.com/10.1007/BF02668782

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VL - 13

JO - Plant Molecular Biology Reporter

JF - Plant Molecular Biology Reporter

IS - 2

ER -

Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

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