Construction and characterization of a bacterial artificial chromosome library of Arabidopsis thaliana

Sangdun Choi, Robert A. Creelman, John E. Mullet, Rod A. Wing

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We constructed an ordered 3,948-clone arabidopsis BAC library. The library has a combined average insert size of 100 kb (n=54). Assuming a haploid genome size of 100,000 kb, the BAC library contains 3.95 haploid genome equivalents with a 98 percent probability of isolating a specific genomic region. The library was screened with five arabidopsis cDNA probes and one tomato probe; all probes hybridized to at least one (and in most cases three) BAC clones in the library. © 1995 Kluwer Academic Publishers.
Original languageEnglish (US)
JournalPlant Molecular Biology Reporter
Issue number2
StatePublished - Jun 1 1995
Externally publishedYes

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