Comparative Proteome and Phosphoproteome Analyses during Cyprid Development of the Barnacle Balanus ( =Amphibalanus ) amphitrite

Yu Zhang, Ying Xu, Shawn M. Arellano, Kang Xiao, Pei-Yuan Qian

Research output: Contribution to journalArticlepeer-review

44 Scopus citations

Abstract

The barnacle Balanus amphitrite (=Amphibalanus amphitrite) is a major marine biofouling invertebrate worldwide. It has a complex life cycle during which the larva (called a nauplius) molts six times before transforming into the cyprid stage. The cyprid stage in B. amphitrite is the critical stage for the larval decision to attach and metamorphose. In this study, proteome and phosphoproteome alterations during cyprid development/aging and upon treatment with the antifouling agent butenolide were examined with a two-dimensional electrophoresis (2-DE) multiplexed fluorescent staining approach. Optimized protein separation strategies, including solution-phase isoelectric fractionation and narrow-pH-range 2-DE, were used in a proteomic analysis. Our results show that the differential regulation of the target proteins is highly dynamic on the levels of both protein expression and posttranslational modification. Two groups of proteins, stress-associated and energy metabolism-related proteins, are differentially expressed during cyprid development. Comparison of the control and treatment groups suggests that butenolide exerts its effects by sustaining the expression levels of these proteins. Altogether, our data suggest that proteins involved in stress regulation and energy metabolism play crucial roles in regulating larval attachment and metamorphosis of B. amphitrite. © 2010 American Chemical Society.
Original languageEnglish (US)
Pages (from-to)3146-3157
Number of pages12
JournalJournal of Proteome Research
Volume9
Issue number6
DOIs
StatePublished - Jun 4 2010
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): SA-C0040, UK-C0016
Acknowledgements: We thank Dr. Kondethimmanahalli Chandramouli for conducting sample fractionation and data analysis, Prof. Donald Choy Chang for providing the Sensor-C3 stably expressed HeLa cells (S3), Dr. Huoming Zhang for proof-reading the manuscript, and the rest of the laboratory team for their constructive comments on this work. This work was supported by a grant from the China Ocean Mineral Resources Research and Development Association (COMRRDA06/07.Sc02), an award (SA-C0040/UK-C0016) of the King Abdullah University of Science and Technology, and the RGC grants (N-HKUST602/09, 662408, and AoE/P-04/04-II) of the government of the Hong Kong Special Administrative Region to PY Qian.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

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