We present and discuss a new experimental setup to perform small angle X-ray scattering and diffraction (SAXSD) of localized liposome colloidal microparticles. A home-built inverted infrared laser tweezers microscope is used to trap, manipulate and aggregate micron-scale liposome particles at single locations inside a 100 microns glass capillary. The micro-focused X-ray and the laser beams are aligned to intersect each other perpendiculary, allowing to associate the X-ray diffraction signal to the micron-sized region of interest inside the capillary. Throughout the laser tweezer setup, using diffractive optical elements implemented on a spatial light modulator, we are able to manipulate small aggregates of colloidal particles (liposomes) and fix them in the optical path of the X-ray beam. We present and discuss first scattering and diffraction experiments on phospholipid liposomes, at the ID 13 microfocus beamline of the European Synchrotron Radiation Facility (ESRF The results demonstrate that we can push the limit of measurable cluster size close to a single liposome.