Clustering of Major Histocompatibility Complex-Class I Molecules in Healthy and Cancer Colon Cells Revealed from Their Nanomechanical Properties

Manola Moretti, Rosanna La Rocca, Michela Perrone Donnorso, Bruno Torre, Claudio Canale, Mario Malerba, Gobind Das, Rosa Sottile, Cinzia Garofalo, Adnane Achour, Klas Kärre, Ennio Carbone, Enzo Di Fabrizio

Research output: Contribution to journalArticlepeer-review

7 Scopus citations


The activation of the T cell mediated immune response relies on the fine interaction between the T cell receptor on the immune cell and the antigen-presenting major histocompatibility complex (MHC) molecules on the membrane surface of antigen-presenting cells. Both the distribution and quantity of MHC/peptide complexes and their adequate morphological presentation affect the activation of the immune cells. In several types of cancer the immune response is down-regulated due to the low expression of MHC-class I (MHC-I) molecules on the cell's surface, and in addition, the mechanical properties of the membrane seem to play a role. Herein, we investigate the distribution of MHC-I molecules and the related nanoscale mechanical environment on the cell surface of two cell lines derived from colon adenocarcinoma and a healthy epithelial colon reference cell line. Atomic force microscopy (AFM) force spectroscopy analysis using an antibody-tagged pyramidal probe specific for MHC-I molecules and a formula that relates the elasticity of the cell to the energy of adhesion revealed the different population distributions of MHC-I molecules in healthy cells compared to cancer cells. We found that MHC-I molecules are significantly less expressed in cancer cells. Moreover, the local elastic modulus is significantly reduced in cancer cells. We speculate that these results might be related to the proven ability of cancer cells to evade the immune system, not only by reducing MHC-I cell surface expression but also by modifying the local mechanical properties affecting the overall morphology of MHC-I synapse presentation to immune cells.
Original languageEnglish (US)
JournalACS Nano
StatePublished - Mar 22 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-03-25
Acknowledged KAUST grant number(s): OCRF-2014-CRG, OCRF-2016-CRG grants
Acknowledgements: We acknowledge financial support from European Project SMD FP7-NMP 2800-SMALL-2 (Proposal No. CP-FP 229375-2), from King Abdullah University of Science and Technology OCRF-2014-CRG and OCRF-2016-CRG grants, and the Italian Ministry of Health under Project Nos. GR-2010-2320665 and GR-2010-2311677. We acknowledge the contribution of F.Cella Zanacchi for the fluorescent microscopy measurements and of G. R. Briola for the drawings in Figure 1,a,b and TOC. We acknowledge G. Stassi and M. Todaro from University of
Palermo for kindly providing us the AG2D line.

ASJC Scopus subject areas

  • General Physics and Astronomy
  • General Materials Science
  • General Engineering


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