Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Guifang Lin; Hui Chen; Bin Tian; Sunish K. Sehgal; Lovepreet Singh; Jingzhong Xie; Nidhi Rawat; Philomin Juliana; Narinder Singh; Sandesh Shrestha; Duane L. Wilson; Hannah Shult; Hyeonju Lee; Adam William Schoen; Vijay K. Tiwari; Ravi P. Singh; Mary J. Guttieri; Harold N. Trick; Jesse Poland; Robert L. Bowden; Guihua Bai; Bikram Gill; Sanzhen Liu

doi:10.1038/s41467-022-30784-9

Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Guifang Lin, Hui Chen, Bin Tian, Sunish K. Sehgal, Lovepreet Singh, Jingzhong Xie, Nidhi Rawat, Philomin Juliana, Narinder Singh, Sandesh Shrestha, Duane L. Wilson, Hannah Shult, Hyeonju Lee, Adam William Schoen, Vijay K. Tiwari, Ravi P. Singh, Mary J. Guttieri, Harold N. Trick, Jesse Poland, Robert L. BowdenGuihua Bai, Bikram Gill, Sanzhen Liu

Biological, Environmental Sciences and Engineering

Research output: Contribution to journal › Article › peer-review

39 Scopus citations

Abstract

The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

Original language	English (US)
Journal	Nature Communications
Volume	13
Issue number	1
DOIs	https://doi.org/10.1038/s41467-022-30784-9
State	Published - Jun 1 2022

Bibliographical note

KAUST Repository Item: Exported on 2022-06-03
Acknowledgements: Funding was provided by the Kansas Wheat Commission award (no. KWC2020-08) to S.L.; the NSF award (no. 1238313) to B.G.; and the NSF award (no. 1822162) to J.P. Additional funding to support this project includes the USDA NIFA awards (nos. 2018-67013-28511 and 2021-67013-35724) to S.L., the NSF awards (nos. 1741090 and 2011500) to S.L., the NSF award (no. 1943155) to N.R. and V.K.T., and the USDA NIFA awards (nos. 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T. We thank Dr. Christopher Toomajian for the discussion, Dr. Alina Akhunova from the Integrative Genomics Facility at Kansas State University for preparation of RNA-Seq libraries, John Raupp for maintaining and sharing Ae. tauschii accessions, Shuangye Wu for DNA extraction and genotyping, Dr. Hongliang Wang for Lr42 genotyping of wheat populations, Andrew Fan Bai for peeling Ae. tauschii seeds, and Taylor Schulden for rust inoculation and phenotyping. This is contribution 20-131-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas.

ASJC Scopus subject areas

General Biochemistry, Genetics and Molecular Biology
General Chemistry
General Physics and Astronomy

Access to Document

10.1038/s41467-022-30784-9

https://repository.kaust.edu.sa/bitstream/10754/678488/1/s41467-022-30784-9.pdf

Cite this

Lin, G., Chen, H., Tian, B., Sehgal, S. K., Singh, L., Xie, J., Rawat, N., Juliana, P., Singh, N., Shrestha, S., Wilson, D. L., Shult, H., Lee, H., Schoen, A. W., Tiwari, V. K., Singh, R. P., Guttieri, M. J., Trick, H. N., Poland, J., ... Liu, S. (2022). Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii. Nature Communications, 13(1). https://doi.org/10.1038/s41467-022-30784-9

@article{49158d2a5ace4df9ab0424859d488772,

title = "Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii",

abstract = "The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.",

author = "Guifang Lin and Hui Chen and Bin Tian and Sehgal, {Sunish K.} and Lovepreet Singh and Jingzhong Xie and Nidhi Rawat and Philomin Juliana and Narinder Singh and Sandesh Shrestha and Wilson, {Duane L.} and Hannah Shult and Hyeonju Lee and Schoen, {Adam William} and Tiwari, {Vijay K.} and Singh, {Ravi P.} and Guttieri, {Mary J.} and Trick, {Harold N.} and Jesse Poland and Bowden, {Robert L.} and Guihua Bai and Bikram Gill and Sanzhen Liu",

note = "KAUST Repository Item: Exported on 2022-06-03 Acknowledgements: Funding was provided by the Kansas Wheat Commission award (no. KWC2020-08) to S.L.; the NSF award (no. 1238313) to B.G.; and the NSF award (no. 1822162) to J.P. Additional funding to support this project includes the USDA NIFA awards (nos. 2018-67013-28511 and 2021-67013-35724) to S.L., the NSF awards (nos. 1741090 and 2011500) to S.L., the NSF award (no. 1943155) to N.R. and V.K.T., and the USDA NIFA awards (nos. 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T. We thank Dr. Christopher Toomajian for the discussion, Dr. Alina Akhunova from the Integrative Genomics Facility at Kansas State University for preparation of RNA-Seq libraries, John Raupp for maintaining and sharing Ae. tauschii accessions, Shuangye Wu for DNA extraction and genotyping, Dr. Hongliang Wang for Lr42 genotyping of wheat populations, Andrew Fan Bai for peeling Ae. tauschii seeds, and Taylor Schulden for rust inoculation and phenotyping. This is contribution 20-131-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas.",

year = "2022",

month = jun,

day = "1",

doi = "10.1038/s41467-022-30784-9",

language = "English (US)",

volume = "13",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Research",

number = "1",

}

Lin, G, Chen, H, Tian, B, Sehgal, SK, Singh, L, Xie, J, Rawat, N, Juliana, P, Singh, N, Shrestha, S, Wilson, DL, Shult, H, Lee, H, Schoen, AW, Tiwari, VK, Singh, RP, Guttieri, MJ, Trick, HN, Poland, J, Bowden, RL, Bai, G, Gill, B & Liu, S 2022, 'Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii', Nature Communications, vol. 13, no. 1. https://doi.org/10.1038/s41467-022-30784-9

TY - JOUR

T1 - Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

AU - Lin, Guifang

AU - Chen, Hui

AU - Tian, Bin

AU - Sehgal, Sunish K.

AU - Singh, Lovepreet

AU - Xie, Jingzhong

AU - Rawat, Nidhi

AU - Juliana, Philomin

AU - Singh, Narinder

AU - Shrestha, Sandesh

AU - Wilson, Duane L.

AU - Shult, Hannah

AU - Lee, Hyeonju

AU - Schoen, Adam William

AU - Tiwari, Vijay K.

AU - Singh, Ravi P.

AU - Guttieri, Mary J.

AU - Trick, Harold N.

AU - Poland, Jesse

AU - Bowden, Robert L.

AU - Bai, Guihua

AU - Gill, Bikram

AU - Liu, Sanzhen

N1 - KAUST Repository Item: Exported on 2022-06-03 Acknowledgements: Funding was provided by the Kansas Wheat Commission award (no. KWC2020-08) to S.L.; the NSF award (no. 1238313) to B.G.; and the NSF award (no. 1822162) to J.P. Additional funding to support this project includes the USDA NIFA awards (nos. 2018-67013-28511 and 2021-67013-35724) to S.L., the NSF awards (nos. 1741090 and 2011500) to S.L., the NSF award (no. 1943155) to N.R. and V.K.T., and the USDA NIFA awards (nos. 2020-67013-32558 and 2020-67013-31460) to N.R. and V.K.T. We thank Dr. Christopher Toomajian for the discussion, Dr. Alina Akhunova from the Integrative Genomics Facility at Kansas State University for preparation of RNA-Seq libraries, John Raupp for maintaining and sharing Ae. tauschii accessions, Shuangye Wu for DNA extraction and genotyping, Dr. Hongliang Wang for Lr42 genotyping of wheat populations, Andrew Fan Bai for peeling Ae. tauschii seeds, and Taylor Schulden for rust inoculation and phenotyping. This is contribution 20-131-J from the Kansas Agricultural Experiment Station, Manhattan, Kansas.

PY - 2022/6/1

Y1 - 2022/6/1

N2 - The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

AB - The wheat wild relative Aegilops tauschii was previously used to transfer the Lr42 leaf rust resistance gene into bread wheat. Lr42 confers resistance at both seedling and adult stages, and it is broadly effective against all leaf rust races tested to date. Lr42 has been used extensively in the CIMMYT international wheat breeding program with resulting cultivars deployed in several countries. Here, using a bulked segregant RNA-Seq (BSR-Seq) mapping strategy, we identify three candidate genes for Lr42. Overexpression of a nucleotide-binding site leucine-rich repeat (NLR) gene AET1Gv20040300 induces strong resistance to leaf rust in wheat and a mutation of the gene disrupted the resistance. The Lr42 resistance allele is rare in Ae. tauschii and likely arose from ectopic recombination. Cloning of Lr42 provides diagnostic markers and over 1000 CIMMYT wheat lines carrying Lr42 have been developed documenting its widespread use and impact in crop improvement.

UR - http://hdl.handle.net/10754/678488

UR - https://www.nature.com/articles/s41467-022-30784-9

U2 - 10.1038/s41467-022-30784-9

DO - 10.1038/s41467-022-30784-9

M3 - Article

C2 - 35650212

SN - 2041-1723

VL - 13

JO - Nature Communications

JF - Nature Communications

IS - 1

ER -

Cloning of the broadly effective wheat leaf rust resistance gene Lr42 transferred from Aegilops tauschii

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this