Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.
|Original language||English (US)|
|Number of pages||4|
|Journal||International Journal of Immunogenetics|
|State||Published - Dec 12 2011|
Bibliographical noteKAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.