Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA

Thennarasu Sugumar, M. Harishankar, G. Dhinakar Raj

Research output: Contribution to journalArticlepeer-review


Full-length cDNA (435 bp) of the interleukin-3(IL-3) gene of the Indian water buffalo was amplified by reverse transcriptase-polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL-3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL-3 also showed very high identity with that of other ruminants, indicating functional cross-reactivity. Structural homology modelling of buffalo IL-3 protein with human IL-3 showed the presence of five helical structures.
Original languageEnglish (US)
Pages (from-to)203-206
Number of pages4
JournalInternational Journal of Immunogenetics
Issue number3
StatePublished - Dec 12 2011
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors thank the Department of Biotechnology (DBT), Government of India, for financial assistance to this Project. ST and MHS were SRFs who worked in this project.


Dive into the research topics of 'Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin-3 cDNA'. Together they form a unique fingerprint.

Cite this