Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos

Michael Oelgeschläger; Hiroki Kuroda; Bruno Reversade; E. M. De Robertis

doi:10.1016/S1534-5807(02)00404-5

Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos

Michael Oelgeschläger, Hiroki Kuroda, Bruno Reversade, E. M. De Robertis

Research output: Contribution to journal › Article › peer-review

122 Scopus citations

Abstract

We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.

Original language	English (US)
Pages (from-to)	219-230
Number of pages	12
Journal	Developmental Cell
Volume	4
Issue number	2
DOIs	https://doi.org/10.1016/S1534-5807(02)00404-5
State	Published - Feb 1 2003
Externally published	Yes

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Generated from Scopus record by KAUST IRTS on 2023-02-15

ASJC Scopus subject areas

Developmental Biology

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10.1016/S1534-5807(02)00404-5

Cite this

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title = "Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos",

abstract = "We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.",

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AU - Reversade, Bruno

AU - De Robertis, E. M.

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AB - We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.

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Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos

Abstract

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ASJC Scopus subject areas

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