Chloroplast Dysfunction Causes Multiple Defects in Cell Cycle Progression in the Arabidopsis crumpled leaf Mutant

Elodie Hudik, Yasushi Yoshioka, SéVerine Domenichini, Mickaël Bourge, Ludivine Soubigout-Taconnat, Christelle Mazubert, Dalong Yi, Sandrine Bujaldon, Hiroyuki Hayashi, Lieven De Veylder, Catherine Bergounioux, Moussa Benhamed, Cécile Raynaud

Research output: Contribution to journalArticlepeer-review

35 Scopus citations

Abstract

The majority of research on cell cycle regulation is focused on the nuclear events that govern the replication and segregation of the genome between the two daughter cells. However, eukaryotic cells contain several compartmentalized organelles with specialized functions, and coordination among these organelles is required for proper cell cycle progression, as evidenced by the isolation of several mutants in which both organelle function and overall plant development were affected. To investigate how chloroplast dysfunction affects the cell cycle, we analyzed the crumpled leaf (crl) mutant of Arabidopsis (Arabidopsis thaliana), which is deficient for a chloroplastic protein and displays particularly severe developmental defects. In the crl mutant, we reveal that cell cycle regulation is altered drastically and that meristematic cells prematurely enter differentiation, leading to reduced plant stature and early endoreduplication in the leaves. This response is due to the repression of several key cell cycle regulators as well as constitutive activation of stress-response genes, among them the cell cycle inhibitor SIAMESE-RELATED5. One unique feature of the crl mutant is that it produces aplastidic cells in several organs, including the root tip. By investigating the consequence of the absence of plastids on cell cycle progression, we showed that nuclear DNA replication occurs in aplastidic cells in the root tip, which opens future research prospects regarding the dialogue between plastids and the nucleus during cell cycle regulation in higher plants.
Original languageEnglish (US)
Pages (from-to)152-167
Number of pages16
JournalPlant Physiology
Volume166
Issue number1
DOIs
StatePublished - Jul 18 2014

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by the Agence Nationale de la Recherche of France (grant no. ANR 2010 JCJC1207 01) and the Interuniversity Attraction Poles Program (grant no. IUAP P7/29 MARS), initiated by the Belgian Science Policy Office, as well as by the facilities and expertise of the Imagif Cell Biology Unit of the Gif Campus, which is supported by the Infrastructures en Biologie Sante et Agronomie, the French National Research Agency, under Investments for the Future programs France-BioImaging Infrastructure (grant no. ANR-10-INSB-04-01), Saclay Plant Sciences (grant no. ANR-10-LABX-0040-SPS), and the Conseil General de l'Essonne.

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