Chinese Spring Wheat (Triticum aestivum L.) Chloroplast Genome: Complete Sequence and Contig Clones

Yasunari Ogihara*, Kazuhiro Isono, Toshio Kojima, Akira Endo, Mitsumasa Hanaoka, Takashi Shiina, Toru Terachi, Shigeko Utsugi, Minoru Murata, Naoki Mori, Shigeo Takumi, Kazuho Ikeo, Takashi Gojobori, Rika Murai, Koji Murai, Yoshihiro Matsuoka, Yukari Ohnishi, Hikaru Tajiri, Koichiro Tsunewaki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

59 Scopus citations


Libraries of plasmid clones covering the entire chloroplast (cp) genome of the common wheat, Triticum aestivum cv. Chinese Spring were constructed and assembled into contig-clones. From these, we obtained the complete nucleotide sequence of wheat chloroplast DNA - a 134,540 bp circular DNA (DDBJ accession no. AB042240) containing four species of ribosomal RNA, 30 genes for 20 species of transfer RNA, and 71 protein coding genes. Additionally, we detected five unidentified open reading frames conserved among grasses. Plasmid clones are available on request.

Original languageEnglish (US)
Pages (from-to)243-253
Number of pages11
JournalPlant Molecular Biology Reporter
Issue number3
StatePublished - 2000
Externally publishedYes

Bibliographical note

Funding Information:
The work was supported in part by a Grant-in-Aid (No. 10309008) from the Ministry of Education, Science, Sports and Culture of Japan.


  • Chinese Spring wheat
  • Chloroplast DNA
  • Clone bank
  • Complete nucleotide sequence
  • Plastome evolution

ASJC Scopus subject areas

  • Molecular Biology
  • Plant Science


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