Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Binh Thanh Vu; Sophia Allaf Shahin; Jonas G. Croissant; Yevhen Fatieiev; Kotaro Matsumoto; Tan Le-Hoang Doan; Tammy Yik; Shirleen Simargi; Altagracia Conteras; Laura Ratliff; Chiara Mauriello Jimenez; Laurence Raehm; Niveen M. Khashab; Jean-Olivier Durand; Carlotta Glackin; Fuyuhiko Tamanoi

doi:10.1038/s41598-018-25573-8

Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Binh Thanh Vu, Sophia Allaf Shahin, Jonas G. Croissant, Yevhen Fatieiev, Kotaro Matsumoto, Tan Le-Hoang Doan, Tammy Yik, Shirleen Simargi, Altagracia Conteras, Laura Ratliff, Chiara Mauriello Jimenez, Laurence Raehm, Niveen M. Khashab, Jean-Olivier Durand, Carlotta Glackin, Fuyuhiko Tamanoi

Research output: Contribution to journal › Article › peer-review

116 Scopus citations

Abstract

New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.

Original language	English (US)
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-25573-8
State	Published - Jun 4 2018

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: This work was supported by a grant from JSPS KAKENHI Grant Number JP15K21764 (to FT) and City of Hope Women’s Cancer Award P30-CA33572 (to CAG). Support by ANR nanoptPDT (to JOD) is gratefully acknowledged. We would like to thank Dr. Yoshihito Uto (Tokushima University) for introducing us to the chicken egg tumor model. We would also like to thank Dr. Sharon Wilczynski, MD, PhD and the Pathology Core at City of Hope for their expertise opinion in verifying the high correlation of our tumor patient samples with tumors produced in the CAM model. Binh Vu was a visiting graduate researcher from Vietnam National University Ho Chi Minh city and was supported by the Center for Global Mentoring (CGM) program.

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41598-018-25573-8

https://repository.kaust.edu.sa/bitstream/10754/628022/2/s41598-018-25573-8.pdf

Cite this

Vu, B. T., Shahin, S. A., Croissant, J. G., Fatieiev, Y., Matsumoto, K., Le-Hoang Doan, T., Yik, T., Simargi, S., Conteras, A., Ratliff, L., Jimenez, C. M., Raehm, L., Khashab, N. M., Durand, J.-O., Glackin, C., & Tamanoi, F. (2018). Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer. Scientific Reports, 8(1). https://doi.org/10.1038/s41598-018-25573-8

@article{7614738df3cd4f279c7503ac581a9a06,

title = "Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer",

abstract = "New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.",

author = "Vu, {Binh Thanh} and Shahin, {Sophia Allaf} and Croissant, {Jonas G.} and Yevhen Fatieiev and Kotaro Matsumoto and {Le-Hoang Doan}, Tan and Tammy Yik and Shirleen Simargi and Altagracia Conteras and Laura Ratliff and Jimenez, {Chiara Mauriello} and Laurence Raehm and Khashab, {Niveen M.} and Jean-Olivier Durand and Carlotta Glackin and Fuyuhiko Tamanoi",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by a grant from JSPS KAKENHI Grant Number JP15K21764 (to FT) and City of Hope Women{\textquoteright}s Cancer Award P30-CA33572 (to CAG). Support by ANR nanoptPDT (to JOD) is gratefully acknowledged. We would like to thank Dr. Yoshihito Uto (Tokushima University) for introducing us to the chicken egg tumor model. We would also like to thank Dr. Sharon Wilczynski, MD, PhD and the Pathology Core at City of Hope for their expertise opinion in verifying the high correlation of our tumor patient samples with tumors produced in the CAM model. Binh Vu was a visiting graduate researcher from Vietnam National University Ho Chi Minh city and was supported by the Center for Global Mentoring (CGM) program.",

year = "2018",

month = jun,

day = "4",

doi = "10.1038/s41598-018-25573-8",

language = "English (US)",

volume = "8",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

number = "1",

}

Vu, BT, Shahin, SA, Croissant, JG, Fatieiev, Y, Matsumoto, K, Le-Hoang Doan, T, Yik, T, Simargi, S, Conteras, A, Ratliff, L, Jimenez, CM, Raehm, L, Khashab, NM, Durand, J-O, Glackin, C & Tamanoi, F 2018, 'Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer', Scientific Reports, vol. 8, no. 1. https://doi.org/10.1038/s41598-018-25573-8

TY - JOUR

T1 - Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

AU - Vu, Binh Thanh

AU - Shahin, Sophia Allaf

AU - Croissant, Jonas G.

AU - Fatieiev, Yevhen

AU - Matsumoto, Kotaro

AU - Le-Hoang Doan, Tan

AU - Yik, Tammy

AU - Simargi, Shirleen

AU - Conteras, Altagracia

AU - Ratliff, Laura

AU - Jimenez, Chiara Mauriello

AU - Raehm, Laurence

AU - Khashab, Niveen M.

AU - Durand, Jean-Olivier

AU - Glackin, Carlotta

AU - Tamanoi, Fuyuhiko

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledgements: This work was supported by a grant from JSPS KAKENHI Grant Number JP15K21764 (to FT) and City of Hope Women’s Cancer Award P30-CA33572 (to CAG). Support by ANR nanoptPDT (to JOD) is gratefully acknowledged. We would like to thank Dr. Yoshihito Uto (Tokushima University) for introducing us to the chicken egg tumor model. We would also like to thank Dr. Sharon Wilczynski, MD, PhD and the Pathology Core at City of Hope for their expertise opinion in verifying the high correlation of our tumor patient samples with tumors produced in the CAM model. Binh Vu was a visiting graduate researcher from Vietnam National University Ho Chi Minh city and was supported by the Center for Global Mentoring (CGM) program.

PY - 2018/6/4

Y1 - 2018/6/4

N2 - New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.

AB - New therapy development is critically needed for ovarian cancer. We used the chicken egg CAM assay to evaluate efficacy of anticancer drug delivery using recently developed biodegradable PMO (periodic mesoporous organosilica) nanoparticles. Human ovarian cancer cells were transplanted onto the CAM membrane of fertilized eggs, resulting in rapid tumor formation. The tumor closely resembles cancer patient tumor and contains extracellular matrix as well as stromal cells and extensive vasculature. PMO nanoparticles loaded with doxorubicin were injected intravenously into the chicken egg resulting in elimination of the tumor. No significant damage to various organs in the chicken embryo occurred. In contrast, injection of free doxorubicin caused widespread organ damage, even when less amount was administered. The lack of toxic effect of nanoparticle loaded doxorubicin was associated with specific delivery of doxorubicin to the tumor. Furthermore, we observed excellent tumor accumulation of the nanoparticles. Lastly, a tumor could be established in the egg using tumor samples from ovarian cancer patients and that our nanoparticles were effective in eliminating the tumor. These results point to the remarkable efficacy of our nanoparticle based drug delivery system and suggests the value of the chicken egg tumor model for testing novel therapies for ovarian cancer.

UR - http://hdl.handle.net/10754/628022

UR - http://www.nature.com/articles/s41598-018-25573-8

UR - http://www.scopus.com/inward/record.url?scp=85048172523&partnerID=8YFLogxK

U2 - 10.1038/s41598-018-25573-8

DO - 10.1038/s41598-018-25573-8

M3 - Article

C2 - 29867159

SN - 2045-2322

VL - 8

JO - Scientific Reports

JF - Scientific Reports

IS - 1

ER -

Chick chorioallantoic membrane assay as an in vivo model to study the effect of nanoparticle-based anticancer drugs in ovarian cancer

Abstract

Bibliographical note

UN SDGs

Access to Document

Other files and links

Fingerprint

Cite this