Characterization of Skeletonema costatum Intracellular Organic Matter and Study of Nanomechanical Properties under Different Solution Conditions

Leonardo Gutierrez, Cyril Aubry, Laure Dramas, Pierre Aimar, Jean-Philippe Croue

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

In the current investigation, a rigorous characterization of the high molecular weight (HMW) compounds of Skeletonema costatum (SKC) intracellular organic matter (IOM), including nanomechanical properties, was conducted. HMW SKC-IOM was characterized as a mixture of polysaccharides, proteins, and lipids. Atomic force microscopy (AFM) provided crucial information of this isolate at a nanoscale resolution. HMW SKC-IOM showed highly responsive to solution chemistry: fully extended chains at low ionic strength, and compressing structures with increasing electrolyte concentration in solution. Interestingly, two regions of different nanomechanical properties were observed: (a) Region #1: located farther from the substrate and showing extended polymeric chains, and (b) Region #2: located
Original languageEnglish (US)
Pages (from-to)154-161
Number of pages8
JournalColloids and Surfaces A: Physicochemical and Engineering Aspects
Volume506
DOIs
StatePublished - Jun 24 2016

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The authors are grateful to the funding from King Abdullah University of Science and Technology (KAUST).

ASJC Scopus subject areas

  • Colloid and Surface Chemistry

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