Characterization of protein-protein interactions: Application to the understanding of peroxisome biogenesis

Sebastien Leon; Ivet Suriapranata; Mingda Yan; Naganand Rayapuram; Amar Patel; Suresh Subramani

doi:10.1385/1-59745-456-7:219

Characterization of protein-protein interactions: Application to the understanding of peroxisome biogenesis

Sebastien Leon, Ivet Suriapranata, Mingda Yan, Naganand Rayapuram, Amar Patel, Suresh Subramani

Biological, Environmental Science and Engineering

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

With the approaching completion of the Pichia pastoris genome, a greater emphasis will have to be placed on the proteome and the protein-protein interactions between its constituents. This chapter discusses methods that have been used for the study of such interactions among both soluble and membrane-associated proteins in peroxisome biogenesis. The procedures are equally applicable to other cellular processes.

Original language	English (US)
Number of pages	19
Journal	Methods in Molecular Biology
Volume	389
DOIs	https://doi.org/10.1385/1-59745-456-7:219
State	Published - Aug 8 2007

Keywords

Co-immunoprecipitation
Mass spectrometry
Membrane protein complexes
Protein complexes
Protein interactions
Soluble protein complexes
Tandem affinity purification
TAP-tag
Yeast two-hybrid

ASJC Scopus subject areas

Molecular Biology
Genetics

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10.1385/1-59745-456-7:219

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AU - Yan, Mingda

AU - Rayapuram, Naganand

AU - Patel, Amar

AU - Subramani, Suresh

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KW - Co-immunoprecipitation

KW - Mass spectrometry

KW - Membrane protein complexes

KW - Protein complexes

KW - Protein interactions

KW - Soluble protein complexes

KW - Tandem affinity purification

KW - TAP-tag

KW - Yeast two-hybrid

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Characterization of protein-protein interactions: Application to the understanding of peroxisome biogenesis

Abstract

Keywords

ASJC Scopus subject areas

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