Characterization of opaque2 modifier QTLs and candidate genes in recombinant inbred lines derived from the K0326Y quality protein maize inbred

David R. Holding, Brenda G. Hunter, John Klingler, Song Wu, Xiaomei Guo, Bryan C. Gibbon, Rongling Wu., Jan Michele Schulze, Rudolf Jung, Brian A. Larkins

Research output: Contribution to journalArticlepeer-review

45 Scopus citations


Quality protein maize (QPM) is a high lysine-containing corn that is based on genetic modification of the opaque2 (o2) mutant. In QPM, modifier genes convert the starchy endosperm of o2 to the vitreous phenotype of wild type maize. There are multiple, unlinked o2 modifier loci (Opm) in QPM and their nature and mode of action are unknown. We previously identified seven Opm QTLs and characterized 16 genes that are differentially up-regulated at a significant level in K0326Y QPM, compared to the starchy endosperm mutant W64Ao2. In order to further characterize these Opm QTLs and the genes up-regulated in K0326Y QPM, we created a population of 314 recombinant inbred lines (RILs) from a cross between K0326Y QPM and W64Ao2. The RILs were characterized for three traits associated with endosperm texture: vitreousness, density and hardness. Genetic linkage analysis of the RIL population confirmed three of the previously identified QTLs associated with o2 endosperm modification in K0326Y QPM. Many of the genes up-regulated in K0326Y QPM showed substantially higher levels of expression in vitreous compared with opaque RILs. These included genes associated with the upstream regulation of the ethylene response pathway, and a gene encoding a regulatory subunit of pyrophosphate-dependent fructose-6-phosphate 1-phosphotransferase, an adaptive enzyme of the glycolytic pathway. © 2010 Springer-Verlag.
Original languageEnglish (US)
Pages (from-to)783-794
Number of pages12
JournalTheoretical and Applied Genetics
Issue number4
StatePublished - Nov 13 2010

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledgements: The research described in this manuscript was supported by grants from the USDA (CSREES 2004-35301-14537), DOE (DE-FG02-96ER20242) and Pioneer Hi-Bred to BAL. We thank Roberto Lizzaraga-Guerra for contributing to the creation of the genetic materials that were used in this analysis.

ASJC Scopus subject areas

  • Genetics
  • Agronomy and Crop Science
  • Biotechnology


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