Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms

Claudius Marondedze; Ludivine Thomas; Christoph A Gehring; Kathryn S. Lilley

doi:10.1186/s12870-019-1750-x

Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms

Claudius Marondedze, Ludivine Thomas, Christoph A Gehring, Kathryn S. Lilley

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

BACKGROUND:RNA-binding proteins (RBPs) are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via RNA-binding domains and these interactions affect RNA availability for translation, RNA stability and turn-over thus affecting both RNA and protein expression essential for developmental and stimulus specific responses. Here we investigate the effect of severe drought stress on the RNA-binding proteome to gain insights into the mechanisms that govern drought stress responses at the systems level. RESULTS:Label-free mass spectrometry enabled the identification 567 proteins of which 150 significantly responded to the drought-induced treatment. A gene ontology analysis revealed enrichment in the

Original language	English (US)
Journal	BMC Plant Biology
Volume	19
Issue number	1
DOIs	https://doi.org/10.1186/s12870-019-1750-x
State	Published - Apr 11 2019

Bibliographical note

KAUST Repository Item: Exported on 2020-10-01
Acknowledged KAUST grant number(s): CRG3–62140383
Acknowledgements: The research was funded by the Office of Competitive Research Grant Program, grant number CRG3–62140383, King Abdullah University of Science and Technology (KAUST).

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10.1186/s12870-019-1750-x

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Cite this

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title = "Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms",

abstract = "BACKGROUND:RNA-binding proteins (RBPs) are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via RNA-binding domains and these interactions affect RNA availability for translation, RNA stability and turn-over thus affecting both RNA and protein expression essential for developmental and stimulus specific responses. Here we investigate the effect of severe drought stress on the RNA-binding proteome to gain insights into the mechanisms that govern drought stress responses at the systems level. RESULTS:Label-free mass spectrometry enabled the identification 567 proteins of which 150 significantly responded to the drought-induced treatment. A gene ontology analysis revealed enrichment in the",

author = "Claudius Marondedze and Ludivine Thomas and Gehring, {Christoph A} and Lilley, {Kathryn S.}",

note = "KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG3–62140383 Acknowledgements: The research was funded by the Office of Competitive Research Grant Program, grant number CRG3–62140383, King Abdullah University of Science and Technology (KAUST).",

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doi = "10.1186/s12870-019-1750-x",

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AU - Marondedze, Claudius

AU - Thomas, Ludivine

AU - Gehring, Christoph A

AU - Lilley, Kathryn S.

N1 - KAUST Repository Item: Exported on 2020-10-01 Acknowledged KAUST grant number(s): CRG3–62140383 Acknowledgements: The research was funded by the Office of Competitive Research Grant Program, grant number CRG3–62140383, King Abdullah University of Science and Technology (KAUST).

PY - 2019/4/11

Y1 - 2019/4/11

N2 - BACKGROUND:RNA-binding proteins (RBPs) are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via RNA-binding domains and these interactions affect RNA availability for translation, RNA stability and turn-over thus affecting both RNA and protein expression essential for developmental and stimulus specific responses. Here we investigate the effect of severe drought stress on the RNA-binding proteome to gain insights into the mechanisms that govern drought stress responses at the systems level. RESULTS:Label-free mass spectrometry enabled the identification 567 proteins of which 150 significantly responded to the drought-induced treatment. A gene ontology analysis revealed enrichment in the

AB - BACKGROUND:RNA-binding proteins (RBPs) are increasingly recognized as regulatory component of post-transcriptional gene expression. RBPs interact with mRNAs via RNA-binding domains and these interactions affect RNA availability for translation, RNA stability and turn-over thus affecting both RNA and protein expression essential for developmental and stimulus specific responses. Here we investigate the effect of severe drought stress on the RNA-binding proteome to gain insights into the mechanisms that govern drought stress responses at the systems level. RESULTS:Label-free mass spectrometry enabled the identification 567 proteins of which 150 significantly responded to the drought-induced treatment. A gene ontology analysis revealed enrichment in the

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Changes in the Arabidopsis RNA-binding proteome reveal novel stress response mechanisms

Abstract

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