TY - JOUR
T1 - Cesarean delivery and hematopoietic stem cell epigenetics in the newborn infant: Implications for future health?
AU - Almgren, Malin
AU - Schlinzig, Titus
AU - Gomez-Cabrero, David
AU - Gunnar, Agneta
AU - Sundin, Mikael
AU - Johansson, Stefan
AU - Norman, Mikael
AU - Ekström, Tomas J.
N1 - Generated from Scopus record by KAUST IRTS on 2021-02-16
PY - 2014/11/1
Y1 - 2014/11/1
N2 - Objective Cesarean section (CS) has been associated with a greater risk for asthma, diabetes, and cancer later in life. Although elective CS continues to rise, it is unclear whether and how it may contribute to compromised future health. Our aim was to investigate the influence of mode of delivery on the epigenetic state in neonatal hematopoietic stem cells. Study Design This was an observational study of 64 healthy, singleton, newborn infants (33 boys) born at term. Cord blood was sampled after elective CS (n = 27) and vaginal delivery. Global deoxyribonucleic acid (DNA) methylation in hematopoietic stem cells (CD34+) was determined by luminometric methylation assay, and genome-wide, locus-specific DNA methylation analysis was performed by Illumina Infinium 450K (Illumina, San Diego, CA), validated by bisulfite-pyrosequencing. Results CD34+ cells from infants delivered by CS were globally more DNA methylated (+2%) than DNA from infants delivered vaginally (P =.02). In relation to mode of delivery, a locus-specific analysis identified 343 loci with a difference in DNA methylation of 10% or greater (P
AB - Objective Cesarean section (CS) has been associated with a greater risk for asthma, diabetes, and cancer later in life. Although elective CS continues to rise, it is unclear whether and how it may contribute to compromised future health. Our aim was to investigate the influence of mode of delivery on the epigenetic state in neonatal hematopoietic stem cells. Study Design This was an observational study of 64 healthy, singleton, newborn infants (33 boys) born at term. Cord blood was sampled after elective CS (n = 27) and vaginal delivery. Global deoxyribonucleic acid (DNA) methylation in hematopoietic stem cells (CD34+) was determined by luminometric methylation assay, and genome-wide, locus-specific DNA methylation analysis was performed by Illumina Infinium 450K (Illumina, San Diego, CA), validated by bisulfite-pyrosequencing. Results CD34+ cells from infants delivered by CS were globally more DNA methylated (+2%) than DNA from infants delivered vaginally (P =.02). In relation to mode of delivery, a locus-specific analysis identified 343 loci with a difference in DNA methylation of 10% or greater (P
UR - https://linkinghub.elsevier.com/retrieve/pii/S0002937814004657
UR - http://www.scopus.com/inward/record.url?scp=84908352037&partnerID=8YFLogxK
U2 - 10.1016/j.ajog.2014.05.014
DO - 10.1016/j.ajog.2014.05.014
M3 - Article
SN - 1097-6868
VL - 211
SP - 502.e1-502.e8
JO - American Journal of Obstetrics and Gynecology
JF - American Journal of Obstetrics and Gynecology
IS - 5
ER -
