Cellular Dynamics Revealed by Digital Holographic Microscopy

P. Marquet; Christian Depeursinge

doi:10.1016/B978-008045046-9.01741-1

Cellular Dynamics Revealed by Digital Holographic Microscopy

P. Marquet^*, Christian Depeursinge

^*Corresponding author for this work

Biological, Environmental Sciences and Engineering

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

1 Scopus citations

Abstract

Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. Specifically, both three-dimensional images of cellular surface morphometry and intracellular refractive index mapping are provided. They result from the hologram numerical reconstruction process, which permits a subwavelength calculation of the phase shift, produced on the transmitted wavefront, by the optically probed cells. Consequently, DHM offers unique possibilities for monitoring biological processes involving rapid and discrete morphological changes, including membrane nano-movements in specific cellular microdomains.

Original language	English (US)
Title of host publication	Encyclopedia of Neuroscience
Publisher	Elsevier Ltd.
Pages	675-683
Number of pages	9
ISBN (Print)	9780080450469
DOIs	https://doi.org/10.1016/B978-008045046-9.01741-1
State	Published - Jan 1 2009

Keywords

Cellular dynamics
Digital holography
Excitotoxocity
Glutamate
Interferometry
Membrane fluctuations
Microscopy
Neuron cell culture
Optical imaging
Red blood cells

ASJC Scopus subject areas

General Neuroscience

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10.1016/B978-008045046-9.01741-1

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title = "Cellular Dynamics Revealed by Digital Holographic Microscopy",

abstract = "Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. Specifically, both three-dimensional images of cellular surface morphometry and intracellular refractive index mapping are provided. They result from the hologram numerical reconstruction process, which permits a subwavelength calculation of the phase shift, produced on the transmitted wavefront, by the optically probed cells. Consequently, DHM offers unique possibilities for monitoring biological processes involving rapid and discrete morphological changes, including membrane nano-movements in specific cellular microdomains.",

keywords = "Cellular dynamics, Digital holography, Excitotoxocity, Glutamate, Interferometry, Membrane fluctuations, Microscopy, Neuron cell culture, Optical imaging, Red blood cells",

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AU - Marquet, P.

AU - Depeursinge, Christian

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N2 - Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. Specifically, both three-dimensional images of cellular surface morphometry and intracellular refractive index mapping are provided. They result from the hologram numerical reconstruction process, which permits a subwavelength calculation of the phase shift, produced on the transmitted wavefront, by the optically probed cells. Consequently, DHM offers unique possibilities for monitoring biological processes involving rapid and discrete morphological changes, including membrane nano-movements in specific cellular microdomains.

AB - Digital holographic microscopy (DHM) is a new optical method taking advantage of coherent properties of light propagation and it provides, without the use of any contrast agent, real-time three-dimensional images of transparent living cells, with an axial sensitivity of a few tens of nanometers. Specifically, both three-dimensional images of cellular surface morphometry and intracellular refractive index mapping are provided. They result from the hologram numerical reconstruction process, which permits a subwavelength calculation of the phase shift, produced on the transmitted wavefront, by the optically probed cells. Consequently, DHM offers unique possibilities for monitoring biological processes involving rapid and discrete morphological changes, including membrane nano-movements in specific cellular microdomains.

KW - Cellular dynamics

KW - Digital holography

KW - Excitotoxocity

KW - Glutamate

KW - Interferometry

KW - Membrane fluctuations

KW - Microscopy

KW - Neuron cell culture

KW - Optical imaging

KW - Red blood cells

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DO - 10.1016/B978-008045046-9.01741-1

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SN - 9780080450469

SP - 675

EP - 683

BT - Encyclopedia of Neuroscience

PB - Elsevier Ltd.

ER -

Cellular Dynamics Revealed by Digital Holographic Microscopy

Abstract

Keywords

ASJC Scopus subject areas

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