Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film

Chor Yong Tay; Haigang Gu; Wen Shing Leong; Haiyang Yu; Hua Qiong Li; Boon Chen Heng; Hosea Tantang; Say Chye Joachim Loo; Lain Jong Li; Lay Poh Tan

doi:10.1016/j.carbon.2009.11.031

Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film

Chor Yong Tay, Haigang Gu, Wen Shing Leong, Haiyang Yu, Hua Qiong Li, Boon Chen Heng, Hosea Tantang, Say Chye Joachim Loo, Lain Jong Li, Lay Poh Tan^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

95 Scopus citations

Abstract

The influences of carboxylic functionalized single-walled carbon nanotubes (SWCNTs) on cell adhesion, spreading and cell lineage commitment of human mesenchymal stem cells (hMSCs) were evaluated. hMSCs were cultured on a thin mesh like layer of SWCNTs with a vertical height of less than 100 nm. The influence of the SWCNT film was significant on the cell spreading and focal adhesion distribution. Cells spread better on a SWCNT film as compared to cover slip (control), resulting in larger cell area and have higher occurrence of filopodia (microspikes) at the cell boundaries. Cytoskeleton arrangement was observed to be less orientated in the cells cultured on a SWCNT film as compared to control. Neurogenic markers such as nestin, glial fibrillary acidic protein and microtubule associated protein 2 genes were transiently upregulated (a process where cellular components, in this case RNA, is increased in response to external variable) over the first week while genes indicative of osteogenesis remained at its nominal level. These results suggest that nano roughness alone is sufficient to modulate cellular behavior and early stage of stem cell lineage commitment without the aid of an induction medium.

Original language	English (US)
Pages (from-to)	1095-1104
Number of pages	10
Journal	Carbon
Volume	48
Issue number	4
DOIs	https://doi.org/10.1016/j.carbon.2009.11.031
State	Published - Apr 2010
Externally published	Yes

Bibliographical note

Funding Information:
The authors would like to thank A∗Star (SERC Grant No.: 072 101 0021 ), Singapore, for the financial support.

ASJC Scopus subject areas

General Chemistry
General Materials Science

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10.1016/j.carbon.2009.11.031

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@article{07022d5b4ab54be3ad3d558a40433bea,

title = "Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film",

abstract = "The influences of carboxylic functionalized single-walled carbon nanotubes (SWCNTs) on cell adhesion, spreading and cell lineage commitment of human mesenchymal stem cells (hMSCs) were evaluated. hMSCs were cultured on a thin mesh like layer of SWCNTs with a vertical height of less than 100 nm. The influence of the SWCNT film was significant on the cell spreading and focal adhesion distribution. Cells spread better on a SWCNT film as compared to cover slip (control), resulting in larger cell area and have higher occurrence of filopodia (microspikes) at the cell boundaries. Cytoskeleton arrangement was observed to be less orientated in the cells cultured on a SWCNT film as compared to control. Neurogenic markers such as nestin, glial fibrillary acidic protein and microtubule associated protein 2 genes were transiently upregulated (a process where cellular components, in this case RNA, is increased in response to external variable) over the first week while genes indicative of osteogenesis remained at its nominal level. These results suggest that nano roughness alone is sufficient to modulate cellular behavior and early stage of stem cell lineage commitment without the aid of an induction medium.",

author = "Tay, {Chor Yong} and Haigang Gu and Leong, {Wen Shing} and Haiyang Yu and Li, {Hua Qiong} and Heng, {Boon Chen} and Hosea Tantang and Loo, {Say Chye Joachim} and Li, {Lain Jong} and Tan, {Lay Poh}",

note = "Funding Information: The authors would like to thank A∗Star (SERC Grant No.: 072 101 0021 ), Singapore, for the financial support. ",

year = "2010",

month = apr,

doi = "10.1016/j.carbon.2009.11.031",

language = "English (US)",

volume = "48",

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journal = "Carbon",

issn = "0008-6223",

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T1 - Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film

AU - Tay, Chor Yong

AU - Gu, Haigang

AU - Leong, Wen Shing

AU - Yu, Haiyang

AU - Li, Hua Qiong

AU - Heng, Boon Chen

AU - Tantang, Hosea

AU - Loo, Say Chye Joachim

AU - Li, Lain Jong

AU - Tan, Lay Poh

N1 - Funding Information: The authors would like to thank A∗Star (SERC Grant No.: 072 101 0021 ), Singapore, for the financial support.

PY - 2010/4

Y1 - 2010/4

N2 - The influences of carboxylic functionalized single-walled carbon nanotubes (SWCNTs) on cell adhesion, spreading and cell lineage commitment of human mesenchymal stem cells (hMSCs) were evaluated. hMSCs were cultured on a thin mesh like layer of SWCNTs with a vertical height of less than 100 nm. The influence of the SWCNT film was significant on the cell spreading and focal adhesion distribution. Cells spread better on a SWCNT film as compared to cover slip (control), resulting in larger cell area and have higher occurrence of filopodia (microspikes) at the cell boundaries. Cytoskeleton arrangement was observed to be less orientated in the cells cultured on a SWCNT film as compared to control. Neurogenic markers such as nestin, glial fibrillary acidic protein and microtubule associated protein 2 genes were transiently upregulated (a process where cellular components, in this case RNA, is increased in response to external variable) over the first week while genes indicative of osteogenesis remained at its nominal level. These results suggest that nano roughness alone is sufficient to modulate cellular behavior and early stage of stem cell lineage commitment without the aid of an induction medium.

AB - The influences of carboxylic functionalized single-walled carbon nanotubes (SWCNTs) on cell adhesion, spreading and cell lineage commitment of human mesenchymal stem cells (hMSCs) were evaluated. hMSCs were cultured on a thin mesh like layer of SWCNTs with a vertical height of less than 100 nm. The influence of the SWCNT film was significant on the cell spreading and focal adhesion distribution. Cells spread better on a SWCNT film as compared to cover slip (control), resulting in larger cell area and have higher occurrence of filopodia (microspikes) at the cell boundaries. Cytoskeleton arrangement was observed to be less orientated in the cells cultured on a SWCNT film as compared to control. Neurogenic markers such as nestin, glial fibrillary acidic protein and microtubule associated protein 2 genes were transiently upregulated (a process where cellular components, in this case RNA, is increased in response to external variable) over the first week while genes indicative of osteogenesis remained at its nominal level. These results suggest that nano roughness alone is sufficient to modulate cellular behavior and early stage of stem cell lineage commitment without the aid of an induction medium.

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Cellular behavior of human mesenchymal stem cells cultured on single-walled carbon nanotube film

Abstract

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ASJC Scopus subject areas

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