Cells and tissue imaging with digital holographic microscopy

Christian D. Depeursinge; Etienne Cuche; Tristan Colomb; Pia Dahlgren; Anca M. Marian; Frederic Monfort; Pierre Marquet; Pierre J. Magistretti

doi:10.1117/12.500899

Cells and tissue imaging with digital holographic microscopy

Christian D. Depeursinge^*, Etienne Cuche, Tristan Colomb, Pia Dahlgren, Anca M. Marian, Frederic Monfort, Pierre Marquet, Pierre J. Magistretti

^*Corresponding author for this work

Research output: Contribution to journal › Conference article › peer-review

6 Scopus citations

Abstract

The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.

Original language	English (US)
Pages (from-to)	134-138
Number of pages	5
Journal	Proceedings of SPIE - The International Society for Optical Engineering
Volume	5143
DOIs	https://doi.org/10.1117/12.500899
State	Published - 2003
Externally published	Yes
Event	Novel Optical Instrumentation for Biomedical Applications - Munich, Germany Duration: Jun 24 2003 → Jun 25 2003

Keywords

Cell culture imaging
Functional imaging
Holography
Microscopy
Phase contrast

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Condensed Matter Physics
Computer Science Applications
Applied Mathematics
Electrical and Electronic Engineering

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@article{203f423fdf2c412e98e9871788d77b63,

title = "Cells and tissue imaging with digital holographic microscopy",

abstract = "The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.",

keywords = "Cell culture imaging, Functional imaging, Holography, Microscopy, Phase contrast",

author = "Depeursinge, {Christian D.} and Etienne Cuche and Tristan Colomb and Pia Dahlgren and Marian, {Anca M.} and Frederic Monfort and Pierre Marquet and Magistretti, {Pierre J.}",

year = "2003",

doi = "10.1117/12.500899",

language = "English (US)",

volume = "5143",

pages = "134--138",

journal = "Proceedings of SPIE - The International Society for Optical Engineering",

issn = "0277-786X",

publisher = "SPIE",

note = "Novel Optical Instrumentation for Biomedical Applications ; Conference date: 24-06-2003 Through 25-06-2003",

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TY - JOUR

T1 - Cells and tissue imaging with digital holographic microscopy

AU - Depeursinge, Christian D.

AU - Cuche, Etienne

AU - Colomb, Tristan

AU - Dahlgren, Pia

AU - Marian, Anca M.

AU - Monfort, Frederic

AU - Marquet, Pierre

AU - Magistretti, Pierre J.

PY - 2003

Y1 - 2003

N2 - The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.

AB - The aim of Optical Digital Holography, applied to cells and tissue imaging, is to provide an accurate 3D imaging of biologic materials, down to the microscopic scale. The method has been developed to yield a very precise determination of cells and tissues morphology. Targeted accuracies are in the sub-micron range and allow for the observation of very small movements and deformations, produced, in particular, by depolarization of excitable cells and their metabolic activities. Direct imaging of tissue structures by the newly developed digital holography is deemed to offer unique investigation means in biology and medicine and attractive diagnostic capabilities.

KW - Cell culture imaging

KW - Functional imaging

KW - Holography

KW - Microscopy

KW - Phase contrast

UR - http://www.scopus.com/inward/record.url?scp=1342331100&partnerID=8YFLogxK

U2 - 10.1117/12.500899

DO - 10.1117/12.500899

M3 - Conference article

AN - SCOPUS:1342331100

SN - 0277-786X

VL - 5143

SP - 134

EP - 138

JO - Proceedings of SPIE - The International Society for Optical Engineering

JF - Proceedings of SPIE - The International Society for Optical Engineering

T2 - Novel Optical Instrumentation for Biomedical Applications

Y2 - 24 June 2003 through 25 June 2003

ER -

Cells and tissue imaging with digital holographic microscopy

Abstract

Keywords

ASJC Scopus subject areas

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