Cell refractive index tomography by digital holographic microscopy

Florian Charrière*, Anca Marian, Frédéric Montfort, Jonas Kuehn, Tristan Colomb, Etienne Cuche, Pierre Marquet, Christian Depeursinge

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

558 Scopus citations


For what we believe to be the first time, digital holographic microscopy is applied to perform optical diffraction tomography of a pollen grain. Transmission phase images with nanometric axial accuracy are numerically reconstructed from holograms acquired for different orientations of the rotating sample; then the three-dimensional refractive index spatial distribution is computed by inverse radon transform. A precision of 0.01 for the refractive index estimation and a spatial resolution in the micrometer range are demonstrated.

Original languageEnglish (US)
Pages (from-to)178-180
Number of pages3
Issue number2
StatePublished - Jan 15 2006
Externally publishedYes

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics


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