CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells

Amal J. Ali, Jasmeen Merzaban, Ayman AbuElela

Research output: Contribution to journalArticlepeer-review


Abstract Selectins play a central role in the trafficking of activated T cells by mediating tethering and rolling on vascular endothelium, acting as a beacon to help navigate them to the site of infection. Here we present a comprehensive binding and in vitro functional analysis of E-selectin ligands expressed on human activated T cells. Using a mass-spectrometric approach we identified a number of glycoproteins that may act as physiological E-selectin ligands on human activated T cells and focused on comparing the role the previously identified well-known E-selectin ligands, PSGL-1 (P-selectin glycoprotein ligand 1) to the newly identified ligand CD44. We show that CD44 from human activated CD4+ and CD8+ T cells binds E-selectin, and that immobilized CD44 mediates tethering and rolling of E-selectin expressing CHO cells via sialylated N-linked glycans. By knocking down CD44 and/or PSGL-1 in primary human activated T cells, our data demonstrate for the first time that CD44 is essential for mediating the rolling over E-selectin and thereby cooperates with PSGL-1 as a major E-selectin ligand on human activated T cells. This has major implications in the development of targeted therapies to combat inflammatory diseases and in transplantation settings. Disclosures No relevant conflicts of interest to declare.
Original languageEnglish (US)
Pages (from-to)3429-3429
Number of pages1
Issue number23
StatePublished - Dec 3 2015

Bibliographical note

KAUST Repository Item: Exported on 2021-12-13

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Immunology
  • Hematology


Dive into the research topics of 'CD44 and PSGL-1 Collaborate in Controlling the Migration of Human Activated T-Cells'. Together they form a unique fingerprint.

Cite this