CcmFC involved in cytochrome c maturation is present in a large sized complex in wheat mitochondria

Philippe Giegé*, Naganand Rayapuram, Etienne H. Meyer, Jean Michel Grienenberger, Géraldine Bonnard

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

23 Scopus citations


In land plant mitochondria, c-type cytochromes are assembled via a mechanism similar to that found in Gram-negative bacteria and different from that used by mitochondria from other eukaryotes. The wheat mitochondrial genome encodes CCM (for cytochrome c maturation) proteins, among them CcmFC, a protein similar to the C-terminal part of the bacterial CcmF. The gene is transcribed into a 1.7 kb transcript at steady state. However, the 3′ termini of the transcript were found to occur upstream of the deduced gene termination codon. This discrepancy was solved by RNA editing that introduces a novel termination codon, thus shortening the reading frame by 27 codons. The processed transcript is translated into a protein integrated in the mitochondrial inner membrane. We also show that the protein is part of a large (700 kDa) protein complex, that possibly represents a cytochrome c assembly complex.

Original languageEnglish (US)
Pages (from-to)165-169
Number of pages5
JournalFEBS Letters
Issue number1-3
StatePublished - Apr 9 2004
Externally publishedYes

Bibliographical note

Funding Information:
This work was supported by the Centre National de la Recherche Scientifique. P.G. was supported by a MC-ERG grant from the European Union. N.R. was supported by CEFIPRA grant 2003-4. E.H.M. was supported by a fellowship from the French Ministère de la Recherche et de la Technologie. We thank A. Klein for technical assistance and Dr. L.J. Sweetlove for critical reading.


  • BN, blue native
  • Blue native
  • CCM, cytochrome c maturation
  • Cytochrome c biogenesis
  • Gene expression
  • Plant mitochondrion
  • RNA editing

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Cell Biology


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