TY - JOUR
T1 - Carotenoids from UV-resistant Antarctic Microbacterium sp. LEMMJ01
AU - Reis-Mansur, Maria Cristina P.P.
AU - Cardoso-Rurr, Janine S.
AU - Silva, Josemar V.Maiworm Abreu
AU - de Souza, Gabriela Rodrigues
AU - Cardoso, Verônica da Silva
AU - Mansoldo, Felipe Raposo Passos
AU - Pinheiro, Yuri
AU - Schultz, Júnia
AU - Lopez Balottin, Luciene B.
AU - da Silva, Antonio Jorge Ribeiro
AU - Lage, Claudia
AU - dos Santos, Elisabete Pereira
AU - Rosado, Alexandre Soares
AU - Vermelho, Alane Beatriz
N1 - Generated from Scopus record by KAUST IRTS on 2021-02-16
PY - 2019/12/1
Y1 - 2019/12/1
N2 - The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
AB - The Microbacterium sp. LEMMJ01 isolated from Antarctic soil does not belong to any of the nearest species identified in the RDP database. Under UV radiation (A, B and C wavebands) the survival fractions of Microbacterium sp. cells were much higher compared with wild-type E. coli K12A15. Especially remarkable for an Antarctic bacterium, an expressive resistance against high UV-B doses was observed. The increased survival of DNA repair-proficient E. coli grown overnight added of 0.1 mg/ml or 1 mg/ml of the whole pigment extract produced by Microbacterium sp. revealed that part of the resistance of Microbacterium sp. against UV-B radiation seems to be connected with photoprotection by its pigments. Scanning electron microscopy revealed that UV-A and UV-B ensued membrane alterations only in E. coli. The APCI-MS fingerprints revealed the diagnostic ions for neurosporene (m/z 580, 566, 522, 538, and 524) synergism for the first time in this bacterium by HPLC-MS/MS analysis. Carotenoids also were devoid of phototoxicity and cytotoxicity effects in mouse cells and in human keratinocytes and fibroblasts.
UR - http://www.nature.com/articles/s41598-019-45840-6
UR - http://www.scopus.com/inward/record.url?scp=85069268991&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-45840-6
DO - 10.1038/s41598-019-45840-6
M3 - Article
SN - 2045-2322
VL - 9
JO - Scientific Reports
JF - Scientific Reports
IS - 1
ER -