Broad-spectrum resistance against multiple PVY-strains by CRSIPR/Cas13 system in Solanum tuberosum crop

Azka Noureen, Muhammad Zuhaib Khan, Imran Amin, Tayyaba Zainab, Nasim Ahmad, Sibtain Haider, Shahid Mansoor

Research output: Contribution to journalArticlepeer-review

11 Scopus citations


Potato virus Y (PVY) is a deadly environmental constraint that damages productivity of potato (Solanum tuberosum) around the globe. One of the major challenges is to develop resistance against PVY. Emerging clustered regularly short palindromic repeat (CRISPR)/Cas systems have the potential to develop resistance against PVY. In the current research, CRISPR-Cas13 has been exploited to target multiple strains of PVYN, PVYO, and PVYNTN. Multiple genes PI, HC-Pro, P3, Cl1, Cl2, and VPg genes of PVY were targeted by CRISPR/Cas13a. Multiplex gRNA cassettes were developed on the conserved regions of the PVY-genes. Three independent CRISPR/Cas13 transgenic potato lines were developed by applying an optimized concentration of trans-ribo zeatin and indole acetic acid at callus development, rooting, and shooting growth stages. The level of resistance in transgenic plants was confirmed through double-antibody sandwich enzyme-linked immunosorbent assay and real-time quantitative PCR. Our results have shown that efficiency of PVY inhibition was positively correlated with the Cas13a/sgRNA expression. Finding provides the specific functionality of Cas13 with specific gRNA cassette and engineering the potential resistance in potato crop against multiple strains of PVY.
Original languageEnglish (US)
Pages (from-to)97-111
Number of pages15
JournalGM crops & food
Issue number1
StatePublished - Jun 2 2022
Externally publishedYes

Bibliographical note

KAUST Repository Item: Exported on 2022-06-08
Acknowledgements: This research was funded by a research grant from Higher Education Commission (HEC-project# 7308) and National Centre for Industrial Biotechnology (NCIB) for establishment of emerging CRISPR/Cas’s techniques in tetraploid Solanum tuberosum. We are thankful to our Collaborator Prof. Magdy M. Mahfouz (King Abdullah University of Science and Technology) for providing Cas13a gene for conducting this study.
This publication acknowledges KAUST support, but has no KAUST affiliated authors.

ASJC Scopus subject areas

  • General Medicine


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