Bottom-up inputs are required for establishment of top-down connectivity onto cortical layer 1 neurogliaform cells.

Leena Ali Ibrahim, Shuhan Huang, Marian Fernandez-Otero, Mia Sherer, Yanjie Qiu, Spurti Vemuri, Qing Xu, Robert Machold, Gabrielle Pouchelon, Bernardo Rudy, Gord Fishell

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

Higher-order projections to sensory cortical areas converge on layer 1 (L1), the primary site for integration of top-down information via the apical dendrites of pyramidal neurons and L1 GABAergic interneurons. Here we investigated the contribution of early thalamic inputs onto L1 interneurons for establishment of top-down connectivity in the primary visual cortex. We find that bottom-up thalamic inputs predominate during L1 development and preferentially target neurogliaform cells. We show that these projections are critical for the subsequent strengthening of top-down inputs from the anterior cingulate cortex onto L1 neurogliaform cells. Sensory deprivation or selective removal of thalamic afferents blocked this phenomenon. Although early activation of the anterior cingulate cortex resulted in premature strengthening of these top-down afferents, this was dependent on thalamic inputs. Our results demonstrate that proper establishment of top-down connectivity in the visual cortex depends critically on bottom-up inputs from the thalamus during postnatal development.
Original languageEnglish (US)
JournalNeuron
DOIs
StatePublished - Sep 3 2021

Bibliographical note

KAUST Repository Item: Exported on 2021-09-06
Acknowledgements: We thank Dr. Anne Takesian and Dr. Chinfei Chen for their valuable comments on the manuscript. We also thank Dr. Shruti Muralidhar for proofreading the manuscript. We thank Dr. Tim Burbridge for demonstrating retinal injections. This work was supported by a Goldenson Foundation grant (FY18) and a Hearst Foundation grant (FY19) (to L.A.I.), grants from the National Institutes of Health (NIH) (MH071679, NS08297, NS074972, and MH095147 to G.F. and P01NS074972, R01NS107257, and R01NS110079 to B.R.), as well as support from the Simons Foundation (SFARI to G.F.).

ASJC Scopus subject areas

  • General Neuroscience

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